ArticleScientific data2016
Genome sequencing of a single tardigrade Hypsibius dujardini individual.
Article in Scientific data, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Modified meiosis in the tardigrade Hypsibius exemplaris maintains heterozygosity across the genome.Genetics · 2026Article
- Modified meiosis in the tardigradebioRxiv : the preprint server for biology · 2026Article
- Candidate Cyanide Resistance Genes in Eutardigrade (Tardigrada) Genomes and KCN Resistance ofInternational journal of molecular sciences · 2026Article
- Life on the dry side: a roadmap to understanding desiccation tolerance and accelerating translational applications.Nature communications · 2025Review
- Single-Animal, Single-Tube RNA Extraction for Comparison of Relative Transcript Levels via qRT-PCR in the Tardigrade Hypsibius exemplaris.Journal of visualized experiments : JoVE · 2025Article
- Single-animal, single-tube RNA extraction for comparison of relative transcript levels via qRT-PCR in the tardigradebioRxiv : the preprint server for biology · 2024Article
- Sexual dimorphism in the tardigrade Paramacrobiotus metropolitanus transcriptome.Zoological letters · 2024Article
- The Evolution of Temperature and Desiccation-Related Protein Families in Tardigrada Reveals a Complex Acquisition of Extremotolerance.Genome biology and evolution · 2024Article
- Article
- Comparative analysis of tardigrade locomotion across life stage, species, and disulfiram treatment.PloS one · 2024Article
- Population Genomics of Pooled Samples: Unveiling Symbiont Infrapopulation Diversity and Host-Symbiont Coevolution.Life (Basel, Switzerland) · 2023Article
- In vivo expression vector derived from anhydrobiotic tardigrade genome enables live imaging in Eutardigrada.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Ultralow-Input Genome Library Preparation for Nanopore Sequencing with Droplet MDA.Methods in molecular biology (Clifton, N.J.) · 2023Article
- How long can tardigrades survive in the anhydrobiotic state? A search for tardigrade anhydrobiosis patterns.PloS one · 2023Article
- Review of extra-embryonic tissues in the closest arthropod relatives, onychophorans and tardigrades.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Trehalose and tardigrade CAHS proteins work synergistically to promote desiccation tolerance.Communications biology · 2022Article
- Deciphering the Biological Enigma-Genomic Evolution Underlying Anhydrobiosis in the Phylum Tardigrada and the ChironomidInsects · 2022Review
- Verification of Hypsibius exemplaris Gąsiorek et al., 2018 (Eutardigrada; Hypsibiidae) application in anhydrobiosis research.PloS one · 2022Article
- Multiomics study of a heterotardigrade, Echinisicus testudo, suggests the possibility of convergent evolution of abundant heat-soluble proteins in Tardigrada.BMC genomics · 2021Article
- Horizontally acquired antibacterial genes associated with adaptive radiation of ladybird beetles.BMC biology · 2021Article
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Tardigrades are ubiquitous microscopic animals that play an important role in the study of metazoan phylogeny. Most terrestrial tardigrades can withstand extreme environments by entering an ametabolic desiccated state termed anhydrobiosis. Due to their small size and the non-axenic nature of laboratory cultures, molecular studies of tardigrades are prone to contamination. To minimize the possibility of microbial contaminations and to obtain high-quality genomic information, we have developed an ultra-low input library sequencing protocol to enable the genome sequencing of a single tardigrade Hypsibius dujardini individual. Here, we describe the details of our sequencing data and the ultra-low input library preparation methodologies.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.