ArticleMolecules (Basel, Switzerland)2016
Influence of Cysteine and Tryptophan Substitution on DNA-Binding Activity on Maize α-Hairpinin Antimicrobial Peptide.
Article in Molecules (Basel, Switzerland), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 37 citations in OpenAlex.
- Exploring Single-Nucleotide Polymorphisms in Primary and Secondary Male Infertility.Medical sciences (Basel, Switzerland) · 2025Article
- Plant Defense Peptides: Exploring the Structure-Function Correlation for Potential Applications in Drug Design and Therapeutics.ACS omega · 2025Review
- Emerging Applications of Plant Antimicrobials in the Food Industry.Current pharmaceutical biotechnology · 2025Review
- The Roadmap of Plant Antimicrobial Peptides Under Environmental Stress: From Farm to Bedside.Probiotics and antimicrobial proteins · 2024Review
- Identification and Characterization of CC-AMP1-like and CC-AMP2-like Peptides inJournal of proteome research · 2024Article
- Article
- Plant Antimicrobial Peptides: Insights into Structure-Function Relationships for Practical Applications.Current issues in molecular biology · 2023Review
- Rational Design of Plant Hairpin-like Peptide EcAMP1: Structural-Functional Correlations to Reveal Antibacterial and Antifungal Activity.Molecules (Basel, Switzerland) · 2022Article
- Article
- Mechanism of Antimicrobial Peptides: Antimicrobial, Anti-Inflammatory and Antibiofilm Activities.International journal of molecular sciences · 2021Review
- Plant antimicrobial peptides: structures, functions, and applications.Botanical studies · 2021Review
- Nigellothionins from Black Cumin (Antibiotics (Basel, Switzerland) · 2021Article
- Tumor-Targeting Peptides: The Functional Screen of Glioblastoma Homing Peptides to the Target Protein FABP3 (MDGI).Cancers · 2020Article
- Defense Peptides From the α-Hairpinin Family Are Components of Plant Innate Immunity.Frontiers in plant science · 2020Review
- Plant Antimicrobial Peptides: State of the Art, In Silico Prediction and Perspectives in the Omics Era.Bioinformatics and biology insights · 2020Review
- Sarconesin II, a New Antimicrobial Peptide Isolated fromMolecules (Basel, Switzerland) · 2019Article
- Diversity of Harpin-Like Defense Peptides from Barnyard Grass (Echinochloa crusgalli L.) Seeds.Doklady. Biochemistry and biophysics · 2019Article
- Characterization of Hydroxyproline-Containing Hairpin-Like Antimicrobial Peptide EcAMP1-Hyp from Barnyard Grass (International journal of molecular sciences · 2018Article
- Efficient Exploitation of Multiple Novel Bacteriocins by Combination of Complete Genome and Peptidome.Frontiers in microbiology · 2018Article
- Investigation of Antimicrobial Peptide Genes Associated with Fungus and Insect Resistance in Maize.International journal of molecular sciences · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
For almost four decades, antimicrobial peptides have been studied, and new classes are being discovered. However, for therapeutic use of these molecules, issues related to the mechanism of action must be answered. In this work, the antimicrobial activity of the hairpinin MBP-1 was studied by the synthesis of two variants, one replacing cysteines and one tryptophan with alanine. Antibacterial activity was abolished in both variants. No membrane disturbance, even in concentrations higher than those required to inhibit the bacteria, was observed in SEM microscopy. The gel retardation assay showed that MBP-1 possesses a higher DNA-binding ability than variants. Finally, molecular modelling showed that the lack of cysteines resulted in structure destabilization and lack of tryptophan resulted in a less flexible peptide, with less solvent assessable surface area, both characteristics that could contribute to absence of activity. In summary, the data here reported add more information about the multiple mechanisms of action of α-hairpinins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.