ArticleProceedings of the National Academy of Sciences of the United States of America2016
Amplification of RNA by an RNA polymerase ribozyme.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 125 papers.
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Who cites it
125 citing papers in PubMed, 264 citations in OpenAlex.
- Exonuclease Ribozyme Encoded in a Circular Genome: On the Initiation of the RNA World.Life (Basel, Switzerland) · 2026Article
- The Emergence of Life in the Light of Evolution.ArXiv · 2026Article
- Periodic Temperature Fluctuations as an Energy Source for RNA Evolution.Life (Basel, Switzerland) · 2026Article
- A universal Fab targeting a conserved U1A-RNA epitope for RNA structure determination by cryo-EM.Nucleic acids research · 2026Article
- The RNA-First Fallacy: Conflating Evolutionary Ancestry with Prebiotic Primacy.Life (Basel, Switzerland) · 2026Article
- Influence of Phosphate Activation Chemistry on the Selection of the Primordial Genetic Alphabet.Journal of the American Chemical Society · 2026Article
- The origin and development of life in an informational dissipative perspective.Theory in biosciences = Theorie in den Biowissenschaften · 2026Article
- A small polymerase ribozyme that can synthesize itself and its complementary strand.Science (New York, N.Y.) · 2026Article
- Growth, Dissolution and Segregation of Genetically Encoded RNA Droplets by Ribozyme Catalysis.Angewandte Chemie (International ed. in English) · 2026Article
- G-quadruplexes self-assembled from nucleotide monomers as stable prepolymer scaffolds in aqueous environments.Scientific reports · 2026Article
- Complex and Messy Prebiotic Chemistry: Obstacles and Opportunities for an RNA World.Life (Basel, Switzerland) · 2026Review
- Brave new RNA world(s): from prebiotic chemistry to gene regulation and RNA technology.Frontiers in genetics · 2026Review
- The Origin of Life and Cellular Systems: A Continuum from Prebiotic Chemistry to Biodiversity.Life (Basel, Switzerland) · 2025Article
- Functional Analysis of Histidine-Dependent Self-Cleaving RNAs Obtained by In Vitro Selection.Chembiochem : a European journal of chemical biology · 2025Article
- Review
- Article
- Trinucleotide substrates under pH-freeze-thaw cycles enable open-ended exponential RNA replication by a polymerase ribozyme.Nature chemistry · 2025Article
- Amino acids catalyse RNA formation under ambient alkaline conditions.Nature communications · 2025Article
- Genetic encoding and expression of RNA origami cytoskeletons in synthetic cells.Nature nanotechnology · 2025Article
- Activation of Caged Functional RNAs by An Oxidative Transformation.Chembiochem : a European journal of chemical biology · 2025Article
65 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In all extant life, genetic information is stored in nucleic acids that are replicated by polymerase proteins. In the hypothesized RNA world, before the evolution of genetically encoded proteins, ancestral organisms contained RNA genes that were replicated by an RNA polymerase ribozyme. In an effort toward reconstructing RNA-based life in the laboratory, in vitro evolution was used to improve dramatically the activity and generality of an RNA polymerase ribozyme by selecting variants that can synthesize functional RNA molecules from an RNA template. The improved polymerase ribozyme is able to synthesize a variety of complex structured RNAs, including aptamers, ribozymes, and, in low yield, even tRNA. Furthermore, the polymerase can replicate nucleic acids, amplifying short RNA templates by more than 10,000-fold in an RNA-catalyzed form of the PCR. Thus, the two prerequisites of Darwinian life-the replication of genetic information and its conversion into functional molecules-can now be accomplished with RNA in the complete absence of proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.