ArticleProceedings of the National Academy of Sciences of the United States of America2016
Regulation of intracellular heme trafficking revealed by subcellular reporters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
What it found
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Who cites it
76 citing papers in PubMed, 122 citations in OpenAlex.
- A conserved vesicular circuit enables metabolic adaptation and maintains systemic heme homeostasis.bioRxiv : the preprint server for biology · 2026Article
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- Mitochondria-ER contacts function as an iron supply hub.Nature cell biology · 2026Article
- The Queen and the Dark Twin: Heme, Protoporphyrin IX, and State Transitions in Liver Metabolism.Molecules (Basel, Switzerland) · 2026Review
- A cell-nonautonomous heme acquisition pathway enables erythroid hemoglobinization under stress.Science (New York, N.Y.) · 2026Article
- Serum Proteomics of Multiple Menstrual Symptoms in Female Athletes: A Pilot Study.FASEB bioAdvances · 2026Article
- Article
- A cell-nonautonomous heme acquisition pathway enables erythroid hemoglobinization under stress.bioRxiv : the preprint server for biology · 2026Article
- Progesterone receptor membrane component 1 (PGRMC1) regulates Heme trafficking through mitochondria-ER junctions.Journal of inorganic biochemistry · 2026Article
- Key roles of GAPDH, Hsp90, and NO in heme trafficking.Journal of inorganic biochemistry · 2026Review
- Comparison of methods for in vivo and ex vivo quantification of iron in murine brain tumors.PloS one · 2026Article
- Blocking extracellular glycine uptake mediated by GlyT1 mitigates protoporphyria.The Journal of clinical investigation · 2025Article
- A novel P450 enzyme assay utilizing an NADPBiotechnology letters · 2025Article
- The heme-regulated inhibitor kinase Hri1 is activated in response to aminolevulinic acid deficiency in Schizosaccharomyces pombe.PLoS genetics · 2025Article
- Update on heme biosynthesis, tissue-specific regulation, heme transport, relation to iron metabolism and cellular energy.Liver international : official journal of the International Association for the Study of the Liver · 2024Review
- Quantitative analysis of heme and hemoglobin for the detection of intravascular hemolysis.Analytica chimica acta · 2024Article
- Impact of MSMEG5257 Deletion onMicroorganisms · 2024Article
- Targeting circulating labile heme as a defense strategy against malaria.Life science alliance · 2024Article
- Trypanosoma cruzi heme responsive gene (TcHRG) plays a central role in orchestrating heme uptake in epimastigotes.The FEBS journal · 2024Article
- Plant-derived foot-and-mouth disease virus antibodies fused to horseradish peroxidase are efficient diagnostic reagents.Plant biotechnology journal · 2024Article
16 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 3 countries.
Funding
Abstract
Heme is an essential prosthetic group in proteins that reside in virtually every subcellular compartment performing diverse biological functions. Irrespective of whether heme is synthesized in the mitochondria or imported from the environment, this hydrophobic and potentially toxic metalloporphyrin has to be trafficked across membrane barriers, a concept heretofore poorly understood. Here we show, using subcellular-targeted, genetically encoded hemoprotein peroxidase reporters, that both extracellular and endogenous heme contribute to cellular labile heme and that extracellular heme can be transported and used in toto by hemoproteins in all six subcellular compartments examined. The reporters are robust, show large signal-to-background ratio, and provide sufficient range to detect changes in intracellular labile heme. Restoration of reporter activity by heme is organelle-specific, with the Golgi and endoplasmic reticulum being important sites for both exogenous and endogenous heme trafficking. Expression of peroxidase reporters in Caenorhabditis elegans shows that environmental heme influences labile heme in a tissue-dependent manner; reporter activity in the intestine shows a linear increase compared with muscle or hypodermis, with the lowest heme threshold in neurons. Our results demonstrate that the trafficking pathways for exogenous and endogenous heme are distinct, with intrinsic preference for specific subcellular compartments. We anticipate our results will serve as a heuristic paradigm for more sophisticated studies on heme trafficking in cellular and whole-animal models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.