ArticleJournal of lipid research2016
Biosynthetic mechanism of very long chain polyunsaturated fatty acids in Thraustochytrium sp. 26185.
Article in Journal of lipid research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 96 citations in OpenAlex.
- Thraustochytrid PUFA synthase ER domains form a stable heterodimer.Journal of structural biology: X · 2026Article
- Acetyl- and malonyl-CoA availability drive EPA selectivity in polyketide synthase-engineered Yarrowia lipolytica.Microbial cell factories · 2026Article
- Genetic Analysis of Polyunsaturated Fatty Acids Biosynthesis Pathway Determines Four Distinct Thraustochytrid Types.Environmental microbiology · 2025Article
- Enhancing fatty acid and omega-3 production in Schizochytrium sp. using developed safe-harboring expression system.Journal of biological engineering · 2024Article
- Comparative Transcriptomic Analysis on the Effect of Sesamol on the Two-Stages Fermentation ofMarine drugs · 2024Article
- Knockout of a PLD gene in Schizochytrium limacinum SR21 enhances docosahexaenoic acid accumulation by modulation of the phospholipid profile.Biotechnology for biofuels and bioproducts · 2024Article
- Molecular cloning and functional analysis of a plastidial ω3 desaturase fromFrontiers in microbiology · 2024Article
- Simultaneous DHA and organic selenium production by Schizochytrium sp.: a theoretical basis.Scientific reports · 2023Article
- Efficient co-production of EPA and DHA by Schizochytrium sp. via regulation of the polyketide synthase pathway.Communications biology · 2022Article
- Functional analysis of the dehydratase domains of the PUFA synthase from Emiliania huxleyi in Escherichia coli and Arabidopsis thaliana.Biotechnology for biofuels and bioproducts · 2022Article
- Application of Marine Microbial Natural Products in Cosmetics.Frontiers in microbiology · 2022Review
- Deciphering and engineering the polyunsaturated fatty acid synthase pathway from eukaryotic microorganisms.Frontiers in bioengineering and biotechnology · 2022Review
- PUFA synthase-independent DHA synthesis pathway in Parietichytrium sp. and its modification to produce EPA and n-3DPA.Communications biology · 2021Article
- Article
- A non-canonical Δ9-desaturase synthesizing palmitoleic acid identified in the thraustochytrid Aurantiochytrium sp. T66.Applied microbiology and biotechnology · 2021Article
- Functional Analysis of an Acyltransferase-Like Domain from Polyunsaturated Fatty Acid Synthase in Thraustochytrium.Microorganisms · 2021Article
- Fatty Acids Derivatives From Eukaryotic Microalgae, Pathways and Potential Applications.Frontiers in microbiology · 2021Review
- Temperature Acclimation of the PicoalgaFrontiers in plant science · 2021Article
- Comparative Transcriptomic Analysis Uncovers Genes Responsible for the DHA Enhancement in the MutantMicroorganisms · 2020Article
- Genome Sequencing and Analysis ofMarine drugs · 2020Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thraustochytrium, a unicellular marine protist, has been used as a commercial source of very long chain PUFAs (VLCPUFAs) such as DHA (22:6n-3). Our recent work indicates coexistence of a Δ4-desaturation-dependent pathway (aerobic) and a polyketide synthase-like PUFA synthase pathway (anaerobic) to synthesize the fatty acids in Thraustochytrium sp. 26185. Heterologous expression of the Thraustochytrium PUFA synthase along with a phosphopantetheinyl transferase in Escherichia coli showed the anaerobic pathway was highly active in the biosynthesis of VLCPUFAs. The amount of Δ4 desaturated VLCPUFAs produced reached about 18% of the total fatty acids in the transformant cells at day 6 in a time course of the induced expression. In Thraustochytrium, the expression level of the PUFA synthase gene was much higher than that of the Δ4 desaturase gene, and also highly correlated with the production of VLCPUFAs. On the other hand, Δ9 and Δ12 desaturations in the aerobic pathway were either ineffective or absent in the species, as evidenced by the genomic survey, heterologous expression of candidate genes, and in vivo feeding experiments. These results indicate that the anaerobic pathway is solely responsible for the biosynthesis for VLCPUFAs in Thraustochytrium.
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