ArticleMicrobial ecology in health and disease2016
Characterisation of probiotic properties in human vaginal lactobacilli strains.
Article in Microbial ecology in health and disease, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed.
- Exploring potential of vaginal Lactobacillus isolates from South African women for enhancing treatment for bacterial vaginosis.PLoS pathogens · 2020Trial
- Characterization of vaginal lactobacilli from South African women toward the development of a biotherapeutic to optimize the vaginal microbiome.Applied and environmental microbiology · 2026Article
- Predicting bacterial vaginosis incidence using artificial neural networks.PLoS computational biology · 2026Article
- One Health Investigation of Stage-Dependent Antimicrobial Resistance Patterns Across Intermediate and Ripened Dairy Matrices: The Tyrovolia-Kopanisti Paradigm.Microorganisms · 2026Article
- Regulatory Effects ofMicroorganisms · 2026Article
- Adhesion Properties and Pathogen Inhibition of Vaginal-Derived Lactobacilli.Probiotics and antimicrobial proteins · 2025Article
- Human breast milk-derived Limosilactobacillus fermentum strains modulate hBD-2 expression against Neisseria meningitidis infection through the TLR-2/p38-MAPK/NF-κB pathway.World journal of microbiology & biotechnology · 2025Article
- Antimicrobial and Anticancer Activities ofNutrients · 2025Article
- Phage Therapy for Urinary Tract Infections: Progress and Challenges Ahead.International urogynecology journal · 2025Review
- Vaginal Candida albicans infections: host-pathogen-microbiome interactions.FEMS microbiology reviews · 2025Review
- Advancements in vaginal microbiota,Microbial cell (Graz, Austria) · 2025Review
- Cutting-edge perspectives on biosurfactants: implications for antimicrobial and biomedical applications.3 Biotech · 2024Review
- Protective Mechanisms of Vaginal Lactobacilli against Sexually Transmitted Viral Infections.International journal of molecular sciences · 2024Review
- Evidence ofMicrobial genomics · 2024Article
- Characterization of Vaginal Lactobacilli with Potential Probiotic Properties Isolated from Healthy Women in Northern Iran.Indian journal of microbiology · 2024Article
- The phenotypic and genotypic antibiotic susceptibility of vaginalIranian journal of microbiology · 2024Article
- The influence of placenta microbiota of normal term pregnant women on immune regulation during pregnancy.BMC pregnancy and childbirth · 2024Article
- Review
- Substances Secreted byInternational journal of molecular sciences · 2023Article
- Article
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13 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundVaginal lactobacilli offer protection against recurrent urinary infections, bacterial vaginosis, and vaginal candidiasis.
objectiveTo characterise the isolated vaginal lactobacilli strains for their probiotic properties and to compare their probiotic potential.
methodsThe Lactobacillus strains were isolated from vaginal samples by conventional culturing and identified by sequencing of the 16S rDNA fragment. Several functional properties were detected (production of hydrogen peroxide and lactic acid; antagonistic activity against Escherichia coli, Candida albicans, Candida glabrata, and Gardnerella vaginalis; auto-aggregation and adhesiveness) as well as safety (haemolytic activity, antibiotic susceptibility, presence of transferrable resistance genes).
resultsA total of 135 vaginal lactobacilli strains of three species, Lactobacillus crispatus (56%), Lactobacillus jensenii (26%), and Lactobacillus gasseri (18%) were characterised using several functional and safety tests. Most of L. crispatus (89%) and L. jensenii (86%) strains produced H2O2. The best lactic acid producers were L. gasseri (18.2±2.2 mg/ml) compared to L. crispatus (15.6±2.8 mg/ml) and L. jensenii (11.6±2.6 mg/ml) (p<0.0001; p<0.0001, respectively). L. crispatus strains showed significantly higher anti-E. coli activity compared to L. jensenii. L. gasseri strains expressed significantly lower anticandidal activity compared to L. crispatus and L. jensenii (p<0.0001). There was no significant difference between the species in antagonistic activity against G. vaginalis. Nearly a third of the strains were able to auto-aggregate while all the tested strains showed a good ability to adhere to HeLa cells. None of the tested lactobacilli caused haemolysis. Although phenotypical resistance was not found to ampicillin, chloramphenicol, erythromycin, gentamycin, tetracycline, and vancomycin, the erm(B), tet(M), and tet(K) were detected in some strains. All strains were resistant to metronidazole, trimethoprim/sulfamethoxazole, and kanamycin.
conclusionsOur study revealed that the production of different antimicrobial metabolites is highly strain-specific and that the metabolites are not correlated with each other. L. crispatus displays better antagonistic activity against E. coli and Candida spp. than L. gasseri and L. jensenii; therefore; a potential probiotic candidate could be found among L. crispatus strains.
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