ArticleScientific reports2016
Selection of suitable reference genes for quantitative RT-PCR normalization in the halophyte Halostachys caspica under salt and drought stress.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed.
- An Efficient Regeneration and Transient Transformation System for the Desert HalophytePlants (Basel, Switzerland) · 2026Article
- Reference Gene Stability inInternational journal of molecular sciences · 2026Article
- Selection of Reference Genes for Expression Normalization by RT-qPCR inCurrent issues in molecular biology · 2024Article
- Selection and validation of reference genes for quantitative real-time PCR of Quercus mongolica Fisch. ex Ledeb under abiotic stresses.PloS one · 2022Article
- The miR169b/NFYA1 module from the halophyteFrontiers in plant science · 2022Article
- Identification of HvLRX, a new dehydration and light responsive gene in Tibetan hulless barley (Hordeum vulgare var. nudum).Genes & genomics · 2021Article
- Selection and Validation of Reference Genes for RT-qPCR Analysis inInternational journal of molecular sciences · 2021Article
- The gut microbiota metabolite urolithin A inhibits NF-κB activation in LPS stimulated BMDMs.Scientific reports · 2021Article
- Identification of valid reference genes for quantitative RT-PCR inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- A 2-Cys peroxiredoxin gene from Tamarix hispida improved salt stress tolerance in plants.BMC plant biology · 2020Article
- Identification of Appropriate Reference Genes for Normalizing miRNA Expression in Citrus Infected byGenes · 2019Article
- Article
- Reference genes selection for Calotropis procera under different salt stress conditions.PloS one · 2019Article
- Selection and validation of reference genes for normalization of qRT-PCR gene expression in wheat (Journal of genetics · 2018Article
- Validation of reference genes for accurate normalization of gene expression with quantitative real-time PCR inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Selection of reference genes for quantitative real-time PCR analysis in halophytic plantPeerJ · 2018Article
- Selection of relatively exact reference genes for gene expression studies in goosegrass (Eleusine indica) under herbicide stress.Scientific reports · 2017Article
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Abstract
The plants are always subjected to various environmental stress, because of plant sessile growth. qRT-PCR is a sensitive and reliable technology, and the normalization of target gene expression with suitable reference genes is very important for obtaining accurate data. Halostachys caspica is an extremely salt-tolerant halophyte belonging to Chenopodiaceae and a good candidate to explore the stress-physiological and molecular mechanism. To get truly the expression profiles of coding genes and miRNAs in H. caspica in response to salt and drought stress using qRT-PCR, suitable reference genes need to be confirmed. In this study, 10 candidate genes including ACT, UBC10, UBC13, TUB2, TUB3, EF1α, 5S rRNA, tRNA, U6 and miR1436 from H. caspica are chosen, and among them, the former nine are commonly used as internal control genes, and miR1436 with high sequence copies is no significant difference expression in high salinity-treated and untreated small RNA libraries of this species. The three softwares are used to analyze expression stability. The results showed that EF1α and TUB3 were the most stable under salt and drought stress, respectively, and UBC10 was the most constant aross all the samples with the both stressed combination. This work will benefit deep studies on abiotic tolerance in H. caspica.
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