ArticleJournal of translational medicine2016
Salivary peptidome profiling for diagnosis of severe early childhood caries.
Article in Journal of translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 2 of them syntheses that pooled it.
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Who cites it
22 citing papers in PubMed, 2 syntheses or guidelines pooled it, 44 citations in OpenAlex.
- Association of salivary proteins with dental caries in children with mixed dentition: a systematic review.European archives of paediatric dentistry : official journal of the European Academy of Paediatric Dentistry · 2025Pooled it
- Assessment of proteomic salivary biomarkers for diagnosis of early childhood caries: a systematic review.Frontiers in oral health · 2025Pooled it
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- [Application of salivary micro-ecosystem in early prevention and control of oral and systemic diseases].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2025Review
- Key Factors Influencing Caries Development in Preschoolers: A Focus on Socio-Demographic, Maternal Health, and Salivary Biomarkers in 3-Year-Olds.Medical science monitor : international medical journal of experimental and clinical research · 2025Observational
- Histatins, proangiogenic molecules with therapeutic implications in regenerative medicine.iScience · 2024Review
- Encapsulation of a novel peptide derived from histatin-1 in liposomes against initial enamel caries in vitro and in vivo.Clinical oral investigations · 2023Article
- Characterization of the salivary microbiome before and after antibiotic therapy via separation technique.Applied microbiology and biotechnology · 2023Article
- Salivary Antimicrobial Peptide Histatin-5 Does Not Display Zn(II)-Dependent or -Independent Activity against Streptococci.ACS infectious diseases · 2023Article
- Peptides in Dentistry: A Scoping Review.Bioengineering (Basel, Switzerland) · 2023Article
- Salivary cystatin S levels in children with early childhood caries in comparison with caries-free children; statistical analysis and machine learning.BMC oral health · 2021Article
- Analysis of salivary proteomic biomarkers for the surveillance of changes in high-risk status of early childhood caries.BMC oral health · 2021Article
- Downregulation of Salivary Proteins, Protective against Dental Caries, in Type 1 Diabetes.Proteomes · 2021Article
- Comparative evaluation of peptidome and microbiota in different types of saliva samples.Annals of translational medicine · 2020Article
- Mutans Streptococci counts from saliva and its protein profile in early childhood caries.Interventional medicine & applied science · 2018Article
- iTRAQ-based quantitative analysis of age-specific variations in salivary proteome of caries-susceptible individuals.Journal of translational medicine · 2018Article
- Comparative salivary proteomics analysis of children with and without dental caries using the iTRAQ/MRM approach.Journal of translational medicine · 2018Article
- Protein-Based Salivary Profiles as Novel Biomarkers for Oral Diseases.Disease markers · 2018Review
- Salivary Protein Roles in Oral Health and as Predictors of Caries Risk.Open life sciences · 2018Article
- Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment.International journal of oral science · 2017Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSevere early childhood caries (s-ECC), which has quite high prevalence among children, is a widespread problem with significant impacts among both developing and developed countries. At present, it is widely known that no early detective techniques and diagnostic tests could have high sensitivity and specificity when using for clinical screening of s-ECC. In this study, we had applied magnetic bead (MB)-based matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) to screen distinctive candidate biomarkers of this disease, so as to establish protein profiles and diagnostic models of s-ECC.
methodsFirstly, we used the technique mentioned above to detect specifically expressed peptides in saliva samples from ten children with s-ECC, separately at the time point of before, 1 and 4 weeks after dental treatment. Then a diagnostic model for s-ECC was established with the K nearest-neighbour method, which was validated in another six children in the next stage of study. After that, linear ion trap-orbitrap-mass spectrometry (LTQ-Orbitrap-MS) was performed to identify which of the proteins in saliva might be the origination of these peptides.
resultsWe found that seven peptide peaks were significantly different when comparing the three time points, among them two were higher, while other five were lower in the pre-treatment s-ECC group compared with post-treatment. The sensitivity and specificity of the diagnostic model we built were both 83.3 %. Two of these peptides were identified to be segments of histatin-1, which was one important secretory protein in saliva.
conclusionsHereby we confirmed that MB-based MALDI-TOF MS is an effective method for screening distinctive peptides from the saliva of junior patients with s-ECC, and histatin-1 may probably be one important candidate biomarker of this common dental disease. These findings might have bright prospect in future in establishing new diagnostic methods for s-ECC.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.