ArticleScientific reports2016
Neutrons describe ectoine effects on water H-bonding and hydration around a soluble protein and a cell membrane.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 83 citations in OpenAlex.
- Osmolyte-Based Formulations for Enhanced Thermal Stability of mRNA Drug Substance: A Systematic Screening and Optimization Study.Pharmaceutical research · 2026Article
- Evaluation of the anti-inflammatory effect of ectoine-leflunomide combination in adjuvant-induced arthritis in rats, and its colonic protective action against leflunomide-induced gastrointestinal injury.Inflammopharmacology · 2026Article
- Enhancing sperm resilience: protective effects of ectoine on post-thaw bovine sperm quality under environmental stress conditions.Journal of animal science · 2026Article
- Review
- Article
- Investigation of the structure of protein-polymer conjugates in solution reveals the impact of protein deuteration and the size of the polymer on its thermal stability.Protein science : a publication of the Protein Society · 2024Article
- Cellular dynamics as a marker of normal-to-cancer transition in human cells.Scientific reports · 2023Article
- Bioactivity profiling of the extremolyte ectoine as a promising protectant and its heterologous production.3 Biotech · 2022Review
- Halomonas sedimenti sp. nov., a Halotolerant Bacterium Isolated from Deep-Sea Sediment of the Southwest Indian Ocean.Current microbiology · 2021Article
- The Effectiveness of the Bacteria Derived Extremolyte Ectoine for the Treatment of Allergic Rhinitis.BioMed research international · 2021Review
- Developing Liquid Rhizobium Inoculants with Enhanced Long-Term Survival, Storage Stability, and Plant Growth Promotion Using Ectoine Additive.Current microbiology · 2021Article
- Ectoine in the Treatment of Irritations and Inflammations of the Eye Surface.BioMed research international · 2021Review
- Protective effect of the extremolytes ectoine and hydroxyectoine in a porcine organ culture.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2020Article
- Role of intracellular water in the normal-to-cancer transition in human cells-insights from quasi-elastic neutron scattering.Structural dynamics (Melville, N.Y.) · 2020Article
- Degradation of the microbial stress protectants and chemical chaperones ectoine and hydroxyectoine by a bacterial hydrolase-deacetylase complex.The Journal of biological chemistry · 2020Article
- The roles and applications of chaotropes and kosmotropes in industrial fermentation processes.World journal of microbiology & biotechnology · 2020Review
- Marine-Derived Compounds with Potential Use as Cosmeceuticals and Nutricosmetics.Molecules (Basel, Switzerland) · 2020Review
- Contribution of mechanosensitive channels to osmoadaptation and ectoine excretion in Halomonas elongata.Extremophiles : life under extreme conditions · 2020Article
- The architecture of the diaminobutyrate acetyltransferase active site provides mechanistic insight into the biosynthesis of the chemical chaperone ectoine.The Journal of biological chemistry · 2020Article
- A New Look into the Mode of Action of Metal-Based Anticancer Drugs.Molecules (Basel, Switzerland) · 2020Article
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Authors and funding
15 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding adaptation to extreme environments remains a challenge of high biotechnological potential for fundamental molecular biology. The cytosol of many microorganisms, isolated from saline environments, reversibly accumulates molar concentrations of the osmolyte ectoine to counterbalance fluctuating external salt concentrations. Although they have been studied extensively by thermodynamic and spectroscopic methods, direct experimental structural data have, so far, been lacking on ectoine-water-protein interactions. In this paper, in vivo deuterium labeling, small angle neutron scattering, neutron membrane diffraction and inelastic scattering are combined with neutron liquids diffraction to characterize the extreme ectoine-containing solvent and its effects on purple membrane of H. salinarum and E. coli maltose binding protein. The data reveal that ectoine is excluded from the hydration layer at the membrane surface and does not affect membrane molecular dynamics, and prove a previous hypothesis that ectoine is excluded from a monolayer of dense hydration water around the soluble protein. Neutron liquids diffraction to atomic resolution shows how ectoine enhances the remarkable properties of H-bonds in water-properties that are essential for the proper organization, stabilization and dynamics of biological structures.
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