ArticleThe Journal of allergy and clinical immunology2017
Assessing basophil activation by using flow cytometry and mass cytometry in blood stored 24 hours before analysis.
Article in The Journal of allergy and clinical immunology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
50 citing papers in PubMed, 91 citations in OpenAlex.
- Can the biomolecular corona induce an allergic reaction?-A proof-of-concept study.Biointerphases · 2021Trial
- Sustained successful peanut oral immunotherapy associated with low basophil activation and peanut-specific IgE.The Journal of allergy and clinical immunology · 2020Trial
- Sustained outcomes in oral immunotherapy for peanut allergy (POISED study): a large, randomised, double-blind, placebo-controlled, phase 2 study.Lancet (London, England) · 2019Trial
- Development of a tool predicting severity of allergic reaction during peanut challenge.Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology · 2018Trial
- A new fluorescent-avidin-based method for quantifying basophil activation in whole blood.The Journal of allergy and clinical immunology · 2017Trial
- The Basophil Activation Test in the Diagnosis of Cow's Milk Allergy in Children: A Narrative Review.Nutrients · 2026Review
- Understanding Prebiotic Allergy: An Evaluation of Basophil Activation Induced by Galacto-Oligosaccharides.Clinical and translational allergy · 2026Article
- Label-free, machine-learning assisted impedance assessment of basophil activation.Sensors and actuators. B, Chemical · 2026Article
- Standardizing a Four-Marker Basophil Activation Test: Influence of Storage Time, Temperature, and IgE Levels.Journal of asthma and allergy · 2026Article
- Basophil Activation Test in IgE-Mediated Wheat Allergy: Diagnostic and Clinical Applications-A Narrative Review.Diagnostics (Basel, Switzerland) · 2025Review
- An HIV-1 gp41 peptide-liposome vaccine elicits neutralizing epitope-targeted antibody responses in healthy individuals.medRxiv : the preprint server for health sciences · 2025Article
- A hand-operated microfluidic sample preparation-to-analysis workflow for simplifying the basophil activation test.Lab on a chip · 2025Article
- Scientific developments in understanding food allergy prevention, diagnosis, and treatment.Frontiers in immunology · 2025Review
- Clinical utility analysis of the Hoxb8 mast cell activation test for the diagnosis of peanut allergy.Allergy · 2025Article
- Enhancing allergy diagnosis: mass spectrometry as a complementary technique to the basophil activation test.Frontiers in allergy · 2025Review
- Applications of basophil activation test in paediatric allergic diseases.The World Allergy Organization journal · 2024Review
- Elucidating allergic reaction mechanisms in response to SARS-CoV-2 mRNA vaccination in adults.Allergy · 2024Article
- A diagnostic approach to IgE-mediated food allergy: A practical algorithm.Journal of food allergy · 2024Article
- Combining avidin with CD63 improves basophil activation test accuracy in classifying peanut allergy.Allergy · 2024Article
- Mass Cytometry in Food Allergy Research.Methods in molecular biology (Clifton, N.J.) · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundBasophil activation tests (BATs) have promise for research and for clinical monitoring of patients with allergies. However, BAT protocols vary in blood anticoagulant used and temperature and time of storage before testing, complicating comparisons of results from various studies.
objectiveWe attempted to establish a BAT protocol that would permit analysis of blood within 24 hours of obtaining the sample.
methodsBlood from 46 healthy donors and 120 patients with peanut allergy was collected into EDTA or heparin tubes, and samples were stored at 4°C or room temperature for 4 or 24 hours before performing BATs.
resultsStimulation with anti-IgE or IL-3 resulted in strong upregulation of basophil CD203c in samples collected in EDTA or heparin, stored at 4°C, and analyzed 24 hours after sample collection. However, a CD63
conclusionBATs to measure upregulation of basophil CD203c and induction of a CD63
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.