ReviewClinical & translational immunology2016
Experimental design considerations in microbiota/inflammation studies.
Review in Clinical & translational immunology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Pooled it
- Contamination-controlled upper gastrointestinal microbiota profiling reveals salivary-duodenal community types linked to opportunistic pathogen carriage and inflammation.Gut microbes · 2025Article
- Navigating ESKAPE Pathogens: Considerations and Caveats for Animal Infection Models Development.ACS infectious diseases · 2024Review
- Neutrophils Mediate Protection Against Colitis and Carcinogenesis by Controlling Bacterial Invasion and IL22 Production by γδ T Cells.Cancer immunology research · 2024Article
- Probiotic supplementation has sex-dependent effects on immune responses in association with the gut microbiota in community-dwelling older adults: a randomized, double-blind, placebo-controlled, multicenter trial.Nutrition research and practice · 2023Article
- Role of microbiota and microbiota-derived short-chain fatty acids in PDAC.Cancer medicine · 2023Review
- Effects of therapeutic levels of dietary antibiotics on the cecal microbiome composition of broiler chickens.Poultry science · 2022Article
- Gut Microbiota-Derived Indole-3-Carboxylate Influences Mucosal Integrity and Immunity Through the Activation of the Aryl Hydrocarbon Receptors and Nutrient Transporters in Broiler Chickens Challenged WithFrontiers in immunology · 2022Article
- Microbiome-Friend or Foe of Pancreatic Cancer?Journal of clinical medicine · 2021Review
- Current challenges and best-practice protocols for microbiome analysis.Briefings in bioinformatics · 2021Review
- Triclocarban exposure exaggerates colitis and colon tumorigenesis: roles of gut microbiota involved.Gut microbes · 2020Article
- Modulation of gut microbiota in healthy rats after exposure to nutritional supplements.Gut microbes · 2020Review
- Gut Microbiota Modulate CD8 T Cell Responses to Influence Colitis-Associated Tumorigenesis.Cell reports · 2020Article
- Microbiota composition modulates inflammation and neointimal hyperplasia after arterial angioplasty.Journal of vascular surgery · 2020Article
- Full-length 16S rRNA gene classification of Atlantic salmon bacteria and effects of using different 16S variable regions on community structure analysis.MicrobiologyOpen · 2019Article
- Probiotics L. acidophilus and B. clausii Modulate Gut Microbiota in Th1- and Th2-Biased Mice to Ameliorate Salmonella Typhimurium-Induced Diarrhea.Probiotics and antimicrobial proteins · 2019Article
- Administration of galacto-oligosaccharide prebiotics in the Flinders Sensitive Line animal model of depression.BMJ open science · 2019Article
- Defining cooperative roles for colonic microbiota and Muc2 mucin in mediating innate host defense against Entamoeba histolytica.PLoS pathogens · 2018Article
- Comparison of anti-inflammatory effect and protein profile between the water extracts from Formosan sambar deer and red deer.Journal of food and drug analysis · 2018Article
- Expansion of commensal fungus Wallemia mellicola in the gastrointestinal mycobiota enhances the severity of allergic airway disease in mice.PLoS pathogens · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There is now convincing evidence that many inflammatory diseases are precipitated, or at least exacerbated, by unfavourable interactions of the host with the resident microbiota. The role of gut microbiota in the genesis and progression of diseases such as inflammatory bowel disease, obesity, metabolic syndrome and diabetes have been studied both in human and in animal, mainly rodent, models of disease. The intrinsic variation in microbiota composition, both within one host over time and within a group of similarly treated hosts, presents particular challenges in experimental design. This review highlights factors that need to be taken into consideration when designing animal trials to investigate the gastrointestinal tract microbiota in the context of inflammation studies. These include the origin and history of the animals, the husbandry of the animals before and during experiments, details of sampling, sample processing, sequence data acquisition and bioinformatic analysis. Because of the intrinsic variability in microbiota composition, it is likely that the number of animals required to allow meaningful statistical comparisons across groups will be higher than researchers have generally used for purely immune-based analyses.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.