ArticleBiotechnology for biofuels2016
Unraveling the microbiome of a thermophilic biogas plant by metagenome and metatranscriptome analysis complemented by characterization of bacterial and archaeal isolates.
Article in Biotechnology for biofuels, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed.
- Proteome-informed interrogation of a methanogenic archaeon links stress response mechanisms with microbial performance and robustness during biomethanation.Engineering microbiology · 2026Article
- Redundancy maintains microbial ecosystem functional potential despite taxonomic shifts between serpentine and nonserpentine soils.Microbial genomics · 2026Article
- Biomethane Production from Stillage: The Role of Temperature, Initial Substrate Load, and Inoculum Variability.ACS omega · 2025Article
- Designing synthetic microbial communities for enhanced anaerobic waste treatment.Applied and environmental microbiology · 2025Review
- Sustainable Biotransformation of Food Waste into Biogas Via Microbial Anaerobic Digestion: A Review.Indian journal of microbiology · 2025Article
- Metagenomics Disentangles Differential Resistome Traits and Risks in Full-Scale Anaerobic Digestion Plants under Ambient, Mesophilic, and Thermophilic Conditions.ACS environmental Au · 2025Article
- Gene network centrality analysis identifies key regulators coordinating day-night metabolic transitions inFrontiers in microbiology · 2025Article
- Exploring microbial diversity in the rhizosphere: a comprehensive review of metagenomic approaches and their applications.3 Biotech · 2024Review
- Phenotypic and genomic characterization ofApplied and environmental microbiology · 2024Article
- Meso- and thermophilic posttreatment of press water coming from a thermophilic municipal solid waste digester.Biotechnology and bioengineering · 2024Article
- Energetically exploiting lignocellulose-rich residues in anaerobic digestion technologies: from bioreactors to proteogenomics.Biotechnology for biofuels and bioproducts · 2023Article
- Uncovering Microbiome Adaptations in a Full-Scale Biogas Plant: Insights from MAG-Centric Metagenomics and Metaproteomics.Microorganisms · 2023Article
- Evaluation of Nanaerobic Digestion as a Mechanism to Explain Surplus Methane Production in Animal Rumina and Engineered Digesters.Environmental science & technology · 2023Article
- Inter-kingdom interactions and stability of methanogens revealed by machine-learning guided multi-omics analysis of industrial-scale biogas plants.The ISME journal · 2023Article
- In silico evaluation of a targeted metaproteomics strategy for broad screening of cellulolytic enzyme capacities in anaerobic microbiome bioreactors.Biotechnology for biofuels and bioproducts · 2022Article
- Phage Genome Diversity in a Biogas-Producing Microbiome Analyzed by Illumina and Nanopore GridION Sequencing.Microorganisms · 2022Article
- Multiplexed Workplace Measurements in Biogas Plants Reveal Compositional Changes in Aerosol Properties.Annals of work exposures and health · 2021Review
- Archaeal community dynamics in biogas fermentation at various temperatures assessed by mcrA amplicon sequencing using different primer pairs.World journal of microbiology & biotechnology · 2021Article
- Management of microbial enzymes for biofuels and biogas production by using metagenomic and genome editing approaches.3 Biotech · 2021Review
- Early response of methanogenic archaea to HMicrobial cell factories · 2021Article
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Abstract
backgroundOne of the most promising technologies to sustainably produce energy and to mitigate greenhouse gas emissions from combustion of fossil energy carriers is the anaerobic digestion and biomethanation of organic raw material and waste towards biogas by highly diverse microbial consortia. In this context, the microbial systems ecology of thermophilic industrial-scale biogas plants is poorly understood.
resultsThe microbial community structure of an exemplary thermophilic biogas plant was analyzed by a comprehensive approach comprising the analysis of the microbial metagenome and metatranscriptome complemented by the cultivation of hydrolytic and acido-/acetogenic Bacteria as well as methanogenic Archaea. Analysis of metagenome-derived 16S rRNA gene sequences revealed that the bacterial genera Defluviitoga (5.5 %), Halocella (3.5 %), Clostridium sensu stricto (1.9 %), Clostridium cluster III (1.5 %), and Tepidimicrobium (0.7 %) were most abundant. Among the Archaea, Methanoculleus (2.8 %) and Methanothermobacter (0.8 %) were predominant. As revealed by a metatranscriptomic 16S rRNA analysis, Defluviitoga (9.2 %), Clostridium cluster III (4.8 %), and Tepidanaerobacter (1.1 %) as well as Methanoculleus (5.7 %) mainly contributed to these sequence tags indicating their metabolic activity, whereas Hallocella (1.8 %), Tepidimicrobium (0.5 %), and Methanothermobacter (<0.1 %) were transcriptionally less active. By applying 11 different cultivation strategies, 52 taxonomically different microbial isolates representing the classes Clostridia, Bacilli, Thermotogae, Methanomicrobia and Methanobacteria were obtained. Genome analyses of isolates support the finding that, besides Clostridium thermocellum and Clostridium stercorarium, Defluviitoga tunisiensis participated in the hydrolysis of hemicellulose producing ethanol, acetate, and H2/CO2. The latter three metabolites are substrates for hydrogentrophic and acetoclastic archaeal methanogenesis.
conclusionsObtained results showed that high abundance of microorganisms as deduced from metagenome analysis does not necessarily indicate high transcriptional or metabolic activity, and vice versa. Additionally, it appeared that the microbiome of the investigated thermophilic biogas plant comprised a huge number of up to now unknown and insufficiently characterized species.
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