ArticleFrontiers in zoology2016
Formation of the germ-disc in spider embryos by a condensation-like mechanism.
Article in Frontiers in zoology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 26 citations in OpenAlex.
- Genome-wide quantitative dissection of an arthropod segmented body plan at single-cell resolution.Communications biology · 2025Article
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- An atlas of spider development at single-cell resolution provides new insights into arthropod embryogenesis.EvoDevo · 2024Article
- Extraembryonic tissue in chelicerates: a review and outlook.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2022Review
- Article
- Virtual spherical-shaped multicellular platform for simulating the morphogenetic processes of spider-like body axis formation.Frontiers in cell and developmental biology · 2022Article
- Systemic paralogy and function of retinal determination network homologs in arachnids.BMC genomics · 2020Article
- Embryonic development and secondary axis induction in the Brazilian white knee tarantula Acanthoscurria geniculata, C. L. Koch, 1841 (Araneae; Mygalomorphae; Theraphosidae).Development genes and evolution · 2020Article
- A SoxB gene acts as an anterior gap gene and regulates posterior segment addition in a spider.eLife · 2018Article
- Recent progress and prospects for advancing arachnid genomics.Current opinion in insect science · 2018Review
- A novel role foreLife · 2017Article
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Authors and funding
1 author at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDetermination of the embryonic body axes is a crucial developmental process in all animals. The establishment of the embryonic axes of spiders has been best studied in the common-house-spider Parasteatoda tepidariorum. Here, anteroposterior (AP) polarity arises during germ disc formation; the centre of the germ-disc marks the future posterior pole, and the rim of the disc the future anterior pole of the spider embryo. The centre of the germ disc is also needed for the formation of the cumulus, a group of migratory cells needed to establish dorsoventral (DV) polarity. Thus, both body axes depend on proper germ disc formation and patterning. However, these processes have not been fully analysed at the cellular and molecular level.
resultsHere I present new techniques to stain the cell membranes/outlines in live and fixed spider embryos. I show that the germ-disc is formed from a regular and contiguous blastoderm and that co-ordinated cell shape changes, rather than migration of single cells, are required to drive germ-disc formation in P. tepidariorum embryos. Furthermore, I show that the rate of cell divisions within the embryonic and extra-embryonic region is not involved in the rapid establishment of the germ-disc. Finally, I show that the process of germ-disc formation is dependent on the initiation of zygotic transcription.
conclusionsThe presented data provide new insights in to the formation of the germ-disc in spider embryos. The establishment of the germ-disc in Parasteatoda embryos is a highly dynamic process that involves wide scale cell-shape changes. While most of the blastodermal cells become cuboidal to form the dense germ-disc, the remaining blastodermal cells stay squamous and develop into huge extra-embryonic, yolk rich cells. In addition, this study shows that the onset of zygotic transcription is needed to establish the germ-disc itself, and that the mid-blastula transition of Parasteatoda tepidariorum embryos is prior to any overt axis establishment.
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