ArticleFrontiers in plant science2016
Evaluation of Reference Genes for Quantitative Real-Time PCR Analysis of the Gene Expression in Laticifers on the Basis of Latex Flow in Rubber Tree (Hevea brasiliensis Muell. Arg.).
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed.
- Molecular Insights into Ammonium Sulfate-Induced Secretome Reprogramming ofMicroorganisms · 2025Article
- Comparative proteome and transcriptome analyses suggest the regulation of starch and sucrose metabolism and rubber biosynthesis pathways in the recovery of tapping panel dryness in rubber tree.BMC plant biology · 2025Article
- Reference Genes Selection and Validation forInternational journal of molecular sciences · 2024Article
- Molecular Genetic Research and Genetic Engineering ofPlants (Basel, Switzerland) · 2023Review
- Identification and validation of reference genes for qRT-PCR based studies in horse gram (Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- RNA Sequencing Reveals Phenylpropanoid Biosynthesis Genes and Transcription Factors forFrontiers in genetics · 2021Article
- Identification and evaluation of suitable reference genes for gene expression analysis in rubber tree leaf.Molecular biology reports · 2020Article
- Selection and Validation of Appropriate Reference Genes for Quantitative RT-PCR Analysis inBioMed research international · 2020Article
- RNA-seq-based selection of reference genes for RT-qPCR analysis of pitaya.FEBS open bio · 2019Article
- Genome-Wide Identification and Characterization of the JAZ Gene Family in Rubber Tree (Frontiers in genetics · 2019Article
- Comparison of Reliable Reference Genes Following Different Hormone Treatments by Various Algorithms for qRT-PCR Analysis ofInternational journal of molecular sciences · 2018Article
- Selection and evaluation of reference genes for qRT-PCR analysis inPlant methods · 2018Article
- Selection and Validation of Appropriate Reference Genes for Quantitative Real-Time PCR Normalization in Staminate and Perfect Flowers of AndromonoeciousFrontiers in plant science · 2017Article
- Transcript Profiling ofFrontiers in plant science · 2017Article
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Abstract
Latex exploitation-caused latex flow is effective in enhancing latex regeneration in laticifer cells of rubber tree. It should be suitable for screening appropriate reference gene for analysis of the expression of latex regeneration-related genes by quantitative real-time PCR (qRT-PCR). In the present study, the expression stability of 23 candidate reference genes was evaluated on the basis of latex flow by using geNorm and NormFinder algorithms. Ubiquitin-protein ligase 2a (UBC2a) and ubiquitin-protein ligase 2b (UBC2b) were the two most stable genes among the selected candidate references in rubber tree clones with differential duration of latex flow. The two genes were also high-ranked in previous reference gene screening across different tissues and experimental conditions. By contrast, the transcripts of latex regeneration-related genes fluctuated significantly during latex flow. The results suggest that screening reference gene during latex flow should be an efficient and effective clue for selection of reference genes in qRT-PCR.
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