ArticleFrontiers in physiology2016
A Novel Chip for Cyclic Stretch and Intermittent Hypoxia Cell Exposures Mimicking Obstructive Sleep Apnea.
Article in Frontiers in physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- Understanding the Impact of Hypoxia on Pulmonary Artery Endothelial Cells in Chronic Thromboembolic Pulmonary Hypertension Patients.International journal of molecular sciences · 2026Article
- A flexible strategy to fabricate trumpet-shaped porous PDMS membranes for organ-on-chip application.Biomicrofluidics · 2024Article
- Innovative three-dimensional models for understanding mechanisms underlying lung diseases: powerful tools for translational research.European respiratory review : an official journal of the European Respiratory Society · 2023Review
- Experimental Models to Study End-Organ Morbidity in Sleep Apnea: Lessons Learned and Future Directions.International journal of molecular sciences · 2022Review
- hLMSC Secretome Affects Macrophage Activity Differentially Depending on Lung-Mimetic Environments.Cells · 2022Article
- Basement membrane properties and their recapitulation in organ-on-chip applications.Materials today. Bio · 2022Review
- Cell-Seeded Biomaterial Scaffolds: The Urgent Need for Unanswered Accelerated Angiogenesis.International journal of nanomedicine · 2022Review
- Fast cycling of intermittent hypoxia in a physiomimetic 3D environment: A novel tool for the study of the parenchymal effects of sleep apnea.Frontiers in pharmacology · 2022Article
- Development of a physiomimetic model of acute respiratory distress syndrome by using ECM hydrogels and organ-on-a-chip devices.Frontiers in pharmacology · 2022Article
- Oxygen Biosensors and Control in 3D Physiomimetic Experimental Models.Antioxidants (Basel, Switzerland) · 2021Review
- Experimental Setting for Applying Mechanical Stimuli to Study the Endothelial Response of Ex Vivo Vessels under Realistic Pathophysiological Environments.Life (Basel, Switzerland) · 2021Article
- Lung Microvascular Niche, Repair, and Engineering.Frontiers in bioengineering and biotechnology · 2020Review
- Technical Feasibility and Physiological Relevance of Hypoxic Cell Culture Models.Frontiers in endocrinology · 2020Review
- Stem Cells, Cell Therapies, and Bioengineering in Lung Biology and Diseases 2017. An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2019Article
- Nonlinear elasticity of the lung extracellular microenvironment is regulated by macroscale tissue strain.Acta biomaterialia · 2019Article
- Differential Oxygenation in Tumor Microenvironment Modulates Macrophage and Cancer Cell Crosstalk: Novel Experimental Setting and Proof of Concept.Frontiers in oncology · 2019Article
- Medium throughput breathing human primary cell alveolus-on-chip model.Scientific reports · 2018Article
- Incorporating mechanical strain in organs-on-a-chip: Lung and skin.Biomicrofluidics · 2018Article
- Gas Partial Pressure in Cultured Cells: Patho-Physiological Importance and Methodological Approaches.Frontiers in physiology · 2018Review
- Role of Cyclooxygenase-2 on Intermittent Hypoxia-Induced Lung Tumor Malignancy in a Mouse Model of Sleep Apnea.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
8 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress and inflammation induced by IH are suggested as main contributors of end-organ dysfunction in OSA patients and animal models. Since the molecular mechanisms underlying these in vivo pathological responses remain poorly understood, implementation of experimental in vitro cell-based systems capable of inducing high-frequency IH would be highly desirable. Here, we describe the design, fabrication, and validation of a versatile chip for subjecting cultured cells to fast changes in gas partial pressure and to cyclic stretch. The chip is fabricated with polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin membrane. Cells cultured on top of the membrane can be subjected to fast changes in oxygen concentration (equilibrium time ~6 s). Moreover, cells can be subjected to cyclic stretch at cardiac or respiratory frequencies independently or simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that hypoxia-inducible factor 1α (HIF-1α) expression was increased in response to both stimuli. Thus, the chip provides a versatile tool for the study of cellular responses to cyclical hypoxia and stretch.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.