ArticleCell reports2016
A Link between Integral Membrane Protein Expression and Simulated Integration Efficiency.
Article in Cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Features of membrane protein sequence direct post-translational insertion.Nature communications · 2024Article
- Expression of Xanthorhodopsin in Escherichia coli.The protein journal · 2023Article
- Molecular basis for the evolved instability of a human G-protein coupled receptor.Cell reports · 2021Article
- Dynamics of Co-translational Membrane Protein Integration and Translocation via the Sec Translocon.Journal of the American Chemical Society · 2020Review
- Emerging Diversity in Lipid-Protein Interactions.Chemical reviews · 2019Review
- Contribution of Cotranslational Folding Defects to Membrane Protein Homeostasis.Journal of the American Chemical Society · 2019Article
- Forces on Nascent Polypeptides during Membrane Insertion and Translocation via the Sec Translocon.Biophysical journal · 2018Article
- A statistical model for improved membrane protein expression using sequence-derived features.The Journal of biological chemistry · 2018Article
- Producing membrane proteins one simulation at a time.The Journal of biological chemistry · 2017Article
- Improving membrane protein expression by optimizing integration efficiency.The Journal of biological chemistry · 2017Article
- Structurally detailed coarse-grained model for Sec-facilitated co-translational protein translocation and membrane integration.PLoS computational biology · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Integral membrane proteins (IMPs) control the flow of information and nutrients across cell membranes, yet IMP mechanistic studies are hindered by difficulties in expression. We investigate this issue by addressing the connection between IMP sequence and observed expression levels. For homologs of the IMP TatC, observed expression levels vary widely and are affected by small changes in protein sequence. The effect of sequence changes on experimentally observed expression levels strongly correlates with the simulated integration efficiency obtained from coarse-grained modeling, which is directly confirmed using an in vivo assay. Furthermore, mutations that improve the simulated integration efficiency likewise increase the experimentally observed expression levels. Demonstration of these trends in both Escherichia coli and Mycobacterium smegmatis suggests that the results are general to other expression systems. This work suggests that IMP integration is a determinant for successful expression, raising the possibility of controlling IMP expression via rational design.
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Registered trials
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