Evidence map›Paper›PMID 27523033›Full record

ArticleJournal of neuroscience methods2016

Liposome-entrapped GABA modulates the expression of nNOS in NG108-15 cells.

Gisele C Vaz, Neeru M Sharma, Hong Zheng, Matthew C Zimmerman, Robson S Santos, Frederic Frezard, Marco A P Fontes, Kaushik P Patel

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of neuroscience methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.8field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

  1. Review
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Gisele C VazDepartment of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Neeru M SharmaDepartment of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States.
Hong ZhengDepartment of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States.
Matthew C ZimmermanDepartment of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States.
Robson S SantosDepartment of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Frederic FrezardDepartment of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Marco A P FontesDepartment of Physiology & Biophysics, Federal University of Minas Gerais, Belo Horizonte, Brazil.
Kaushik P PatelDepartment of Cellular and Integrative Physiology, University of Nebraska Medical Center, Omaha, NE 68198-5850, United States. Electronic address: kpatel@unmc.edu.
Universidade Federal de Minas Gerais · BRUniversity of Nebraska Medical Center · US

Funding

The Role of Excitatory Input into the PVN on Increased Sympathetic Drive in HeartP01HL062222 · NHLBI · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI SCHULTZ, HAROLD D · 1999 to 2019
$32.2M
Research Pilot Project ProgramP30GM106397 · NIGMS · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI JOHNSON, KEITH R · 2013 to 2017
$4.9M
Exercise training improves erectile dysfunction in diabetes: role of central mechR01DK082956 · NIDDK · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI PATEL, KAUSHIK P · 2009 to 2012
$1.3M
NHLBI NIH HHS P01 HL062222NHLBI NIH HHS R01 HL124104NIDDK NIH HHS R01 DK082956NIGMS NIH HHS P30 GM106397
6 · The paper itself

Abstract

backgroundLiposomes are concentric lipid vesicles that allow a sustained release of entrapped substances. GABA (γ-aminobutyric acid) is the most prevalent inhibitory neurotransmitter in the central nervous system. NEW

methodUsing GABA-containing liposomes (GL) prepared by the freeze-thawing method, we determined the effect of sustained release of GABA on expression of neuronal nitric oxide synthase (nNOS) and GABA

resultsNeuronal cell line NG108-15 treated with different doses of GL during 24h showed an increase in expression of GABA COMPARISON WITH EXISTING

methodsThis is first demonstration for the development of GL that allows sustained slow release of this neurotransmitter.

conclusionThese results suggest that a slow release of GABA can change the expression of nNOS possibly via alteration in PIN levels in neuronal cells.

Indexed as

AnimalsCell Line, TumorCREB-Binding ProteinCytoplasmic DyneinsDose-Response Relationship, DrugDrug Delivery Systemsgamma-Aminobutyric AcidGene Expression Regulation, NeoplasticGlutamic AcidLiposomesMiceNeuroblastomaNitric Oxide Synthase Type INitritesRatsReceptors, GABA-ACREB-Binding ProteinCytoplasmic DyneinsDynll1 protein, ratgamma-Aminobutyric AcidGlutamic AcidLiposomesNitric Oxide Synthase Type INitritesReceptors, GABA-ARNA, Small InterferingGABALiposomeNG108-15nNOSPIN

Identifiers

PMID27523033
PMCPMC5075514
OpenAlexW2516706294

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.