ArticleInsect biochemistry and molecular biology2016
Multifaceted biological insights from a draft genome sequence of the tobacco hornworm moth, Manduca sexta.
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80 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Chromosomal-level Genome Assembly of the Coffee Bee Hawk Moth Reveals the Evolution of Chromosomes and the Molecular Basis of Distinct Phenotypes.Genome biology and evolution · 2023Article
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- Genome-wide identification, classification, and expression profiling of serine esterases and other esterase-related proteins in the tobacco hornworm, Manduca sexta.Insect science · 2023Article
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- Parallel evolution of opsin visual pigments in hawkmoths by tuning of spectral sensitivities during transition from a nocturnal to a diurnal ecology.The Journal of experimental biology · 2022Article
- Proteomic analysis of pharate pupal molting fluid from the tobacco hornworm, Manduca sexta.Insect biochemistry and molecular biology · 2022Article
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114 authors.
Michael R KanostDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA. Electronic address: Kanost@ksu.edu.
Estela L ArreseDepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
Xiaolong CaoDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Yun-Ru ChenBoyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY, 14853, USA.
Sanjay ChellapillaKSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Marian R GoldsmithBiological Sciences Department, University of Rhode Island, Kingston, RI, 02881, USA.
Ewald Grosse-WildeMax Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
David G HeckelDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
Nicolae HerndonKSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Haobo JiangDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Alexie PapanicolaouHawkesbury Institute for the Environment, Western Sydney University, Richmond, NSW, 2753, Australia.
Jiaxin QuHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Jose L SoulagesDepartment of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, 74078, USA.
Heiko VogelDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
James WaltersDepartment of Ecology and Evolutionary Biology, Univ. Kansas, Lawrence, KS, 66045, USA.
Robert M WaterhouseDepartment of Genetic Medicine and Development, University of Geneva Medical School, rue Michel-Servet 1, 1211, Geneva, Switzerland; Swiss Institute of Bioinformatics, rue Michel-Servet 1, 1211, Geneva, Switzerland; Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, 32 Vassar Street, Cambridge, MA, 02139, USA; The Broad Institute of MIT and Harvard, Cambridge, 415 Main Street, MA, 02142, USA.
Seung-Joon AhnDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
Francisca C AlmeidaDepartament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
Chunju AnDepartment of Entomology, China Agricultural University, Beijing, China.
Peshtewani AqrawiHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Anne BretschneiderDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
William B BryantDivision of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Sascha BucksMax Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
Hsu ChaoHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Germain ChevignonInstitut de Recherche sur la Biologie de l'Insecte, UMR CNRS 7261, UFR Sciences et Techniques, Université François-Rabelais, Tours, France.
Jayne M ChristenDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
David F ClarkeCSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
Neal T DittmerDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
Laura C F FergusonDepartment of Zoology, South Parks Road, Oxford, UK.
Spyridoula GaravelouCentre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Karl H J GordonCSIRO Health and Biosecurity, Clunies Ross St, Acton, ACT, 2601, Australia.
Ramesh T GunaratnaDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Yi HanHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Frank HauserCenter for Functional and Comparative Insect Genomics, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-21oo, Copenhagen, Denmark.
Yan HeDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Hanna Heidel-FischerDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
Ariana HirshDepartment of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
Yingxia HuDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Hongbo JiangKey Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400715, China.
Divya KalraHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Christian KlinnerMax Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
Christopher KönigMax Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
Christie KovarHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Ashley R KrollDepartment of Biology, Reed College, Portland, OR, 97202, USA.
Suyog S KuwarDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
Sandy L LeeHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Rüdiger LehmanFraunhofer Institute for Molecular Biology and Applied Ecology (IME), Bioresources Project Group, Winchesterstrasse 2, 35394, Gießen, Germany.
Kai LiCollege of Chemistry, Chemical Engineering, and Biotechnology, Donghua University, Shanghai, 201620, China.
Zhaofei LiCollege of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Hanquan LiangMcDermott Center for Human Growth and Development, UT Southwestern Medical Center, 5323 Harry Hines Blvd., Dallas, TX, 75390, USA.
Shanna LovelaceDepartment of Biological Sciences, University of Southern Maine, Portland, ME, 04104, USA.
Zhiqiang LuCollege of Plant Protection, Northwest A&F University, Yangling, Shaanxi, 712100, China.
Jennifer H MansfieldDepartment of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
Kyle J McCullochDepartment of Ecology and Evolutionary Biology, University of California, Irvine, CA, 92697, USA.
Tittu MathewHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Brian MortonDepartment of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
Donna M MuznyHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
David NeunemannDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
Fiona OngeriHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Yannick PauchetDepartment of Entomology, Max Planck Institute for Chemical Ecology, Hans-Knoell-Strasse 8, 07745, Jena, Germany.
Ling-Ling PuHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Ioannis PyrousisCentre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
Xiang-Jun RaoSchool of Plant Protection, Anhui Agricultural University, Hefei, Anhui, China.
Amanda ReddingDepartment of Biology, University of Rochester, Rochester, NY, 14627, USA.
Charles RoeselDepartment of Marine and Environmental Sciences, Northeastern University, Boston, MA, 02115, USA.
Alejandro Sanchez-GraciaDepartament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
Sarah SchaackDepartment of Biology, Reed College, Portland, OR, 97202, USA.
Aditi ShuklaDepartment of Biology, Barnard College, Columbia University, 3009 Broadway, New York, NY, 10027, USA.
Guillaume TetreauDepartment of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA.
Yang WangDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Guang-Hua XiongState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Walther TrautInstitut fuer Biologie, Universitaet Luebeck, D-23538, Luebeck, Germany.
Tom K WalshCSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
Kim C WorleyHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Di WuDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
Wenbi WuDivision of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Yuan-Qing WuHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Xiufeng ZhangDepartment of Entomology and Plant Pathology, Oklahoma State University, Stillwater, OK, 74078, USA.
Zhen ZouState Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China.
Hannah ZuckerNeuroscience Program, Hamilton College, Clinton, NY, 13323, USA.
Adriana D BriscoeDepartment of Ecology and Evolutionary Biology, University of California, Irvine, CA, 92697, USA.
Thorsten BurmesterInstitute of Zoology, University of Hamburg, Germany.
Rollie J ClemDivision of Biology, Kansas State University, Manhattan, KS, 66506, USA.
René FeyereisenDepartment of Crop Protection, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
Cornelis J P GrimmelikhuijzenCenter for Functional and Comparative Insect Genomics, Department of Biology, University of Copenhagen, Universitetsparken 15, DK-21oo, Copenhagen, Denmark.
Stavros J HamodrakasDepartment of Cell Biology and Biophysics, Faculty of Biology, University of Athens, Athens, Greece.
Bill S HanssonMax Planck Institute for Chemical Ecology, Department of Evolutionary Neuroethology, Hans-Knoell-Strasse, 8, D-07745, Jena, Germany.
Elisabeth HuguetInstitut de Recherche sur la Biologie de l'Insecte, UMR CNRS 7261, UFR Sciences et Techniques, Université François-Rabelais, Tours, France.
Lars S JermiinCSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
Que LanDepartment of Entomology, University of Wisconsin, Madison, USA.
Herman K LehmanBiology Department and Neuroscience Program, Hamilton College, Clinton, NY, 13323, USA.
Marce LorenzenDept. Entomology, North Carolina State Univ., Raleigh, NC, 27695, USA.
Hans MerzendorferUniversity of Siegen, School of Natural Sciences and Engineering, Institute of Biology - Molecular Biology, Adolf-Reichwein-Strasse. 2, AR-C3010, 57076 Siegen, Germany.
Ioannis MichalopoulosCentre of Systems Biology, Biomedical Research Foundation, Academy of Athens, Athens, Greece.
David B MortonDepartment of Integrative Biosciences, School of Dentistry, BRB421, L595, Oregon Health & Science University, 3181 SW Sam Jackson Park Rd., Portland, OR, 97239, USA.
Subbaratnam MuthukrishnanDepartment of Biochemistry and Molecular Biophysics, Kansas State University, Manhattan, KS, 66506, USA.
John G OakeshottCSIRO Land and Water, Clunies Ross St, Acton, ACT, 2601, Australia.
Will PalmerDepartment of Genetics, University of Cambridge, Downing St, Cambridge, CB2 3EH, UK.
Yoonseong ParkDepartment of Entomology, Kansas State University, Manhattan, KS, 66506, USA.
A Lorena PassarelliDivision of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Julio RozasDepartament de Genètica and Institut de Recerca de la Biodiversitat (IRBio), Universitat de Barcelona, Barcelona, Spain.
Lawrence M SchwartzDepartment of Biology, University of Massachusetts, Amherst, MA, 01003, USA.
Wendy SmithDepartment of Biology, Northeastern University, Boston, MA, 02115, USA.
Agnes SouthgateDepartment of Biology, College of Charleston, Charleston, SC, 29424, USA.
Andreas VilcinskasInstitute for Insect Biotechnology, Justus-Liebig-University, Heinrich-Buff-Ring 26-32, 35392, Giessen, Germany.
Richard VogtDepartment of Biological Sciences, University of South Carolina, Columbia, SC, 29205, USA.
Ping WangDepartment of Entomology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA.
John WerrenDepartment of Biology, University of Rochester, Rochester, NY, 14627, USA.
Xiao-Qiang YuUniversity of Missouri-Kansas City, 5007 Rockhill Road, Kansas City, MO, 64110, USA.
Jing-Jiang ZhouDepartment of Biological Chemistry and Crop Protection, Rothamsted Research, Harpenden, Herts, AL5 2JQ, UK.
Susan J BrownKSU Bioinformatics Center, Division of Biology, Kansas State University, Manhattan, KS, 66506, USA.
Steven E SchererHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Stephen RichardsHuman Genome Sequencing Center, Baylor College of Medicine, 1 Baylor Plaza, Houston, TX, 77030, USA.
Gary W BlissardBoyce Thompson Institute at Cornell University, Tower Road, Ithaca, NY, 14853, USA.
Funding
Initiation and Regulation of Prophenoloxidase ActivationR01GM058634 · NIGMS · OKLAHOMA STATE UNIVERSITY STILLWATER · PI JIANG, HAOBO · 1999 to 2022
$4.0MSupplement 2 to promote diversity in health related res.: protease systems in insR37GM041247 · NIGMS · KANSAS STATE UNIVERSITY · PI KANOST, MICHAEL R · 2011 to 2020
$3.8MMobilization of Triacylglycerol Stores in InsectsR01GM064677 · NIGMS · OKLAHOMA STATE UNIVERSITY STILLWATER · PI ARRESE, ESTELA L. · 2002 to 2018
$3.2MSERINE PROTEINASE INHIBITORS IN INSECTSR01GM041247 · NIGMS · KANSAS STATE UNIVERSITY · PI KANOST, MICHAEL R · 1994 to 2010
$3.1MArbovirus midgut escape mechanismsR01AI091972 · NIAID · KANSAS STATE UNIVERSITY · PI FRANZ, ALEXANDER W E, PASSARELLI, A. LORENA · 2012 to 2015
$2.6MEffects of Blood Meal on Cellular Immunity in MosquitoesF32AI104154 · NIAID · KANSAS STATE UNIVERSITY · PI BRYANT, WILLIAM BART · 2013 to 2014
$115kNIAID NIH HHS F32 AI104154NIAID NIH HHS R01 AI091972NIGMS NIH HHS R01 GM041247NIGMS NIH HHS R01 GM058634NIGMS NIH HHS R01 GM064677NIGMS NIH HHS R37 GM041247
Abstract
Manduca sexta, known as the tobacco hornworm or Carolina sphinx moth, is a lepidopteran insect that is used extensively as a model system for research in insect biochemistry, physiology, neurobiology, development, and immunity. One important benefit of this species as an experimental model is its extremely large size, reaching more than 10 g in the larval stage. M. sexta larvae feed on solanaceous plants and thus must tolerate a substantial challenge from plant allelochemicals, including nicotine. We report the sequence and annotation of the M. sexta genome, and a survey of gene expression in various tissues and developmental stages. The Msex_1.0 genome assembly resulted in a total genome size of 419.4 Mbp. Repetitive sequences accounted for 25.8% of the assembled genome. The official gene set is comprised of 15,451 protein-coding genes, of which 2498 were manually curated. Extensive RNA-seq data from many tissues and developmental stages were used to improve gene models and for insights into gene expression patterns. Genome wide synteny analysis indicated a high level of macrosynteny in the Lepidoptera. Annotation and analyses were carried out for gene families involved in a wide spectrum of biological processes, including apoptosis, vacuole sorting, growth and development, structures of exoskeleton, egg shells, and muscle, vision, chemosensation, ion channels, signal transduction, neuropeptide signaling, neurotransmitter synthesis and transport, nicotine tolerance, lipid metabolism, and immunity. This genome sequence, annotation, and analysis provide an important new resource from a well-studied model insect species and will facilitate further biochemical and mechanistic experimental studies of many biological systems in insects.
Indexed as
Gene ExpressionGenome, InsectAnimalsGene Expression ProfilingLarvaManducaPupaSequence Analysis, DNASyntenyInnate immunityInsectInsect biochemistryLepidopteraMothSyntenyTobacco hornworm
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