Evidence map›Paper›PMID 27522520›Full record

ArticlePlant cell reports2016

Virus-induced gene silencing (VIGS)-mediated functional characterization of two genes involved in lignocellulosic secondary cell wall formation.

Shashank K Pandey, Akula Nookaraju, Takeshi Fujino, Sivakumar Pattathil, Chandrashekhar P Joshi

Abstract read
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In one paragraph

Article in Plant cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
4.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Understanding thePlants (Basel, Switzerland) · 2022
    Review
  4. Genetic Modification ofFrontiers in plant science · 2021
    Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 2 countries.

Shashank K PandeyDepartment of Bioenergy Science and Technology, Chonnam National University, Gwangju, 500-757, South Korea.
Akula NookarajuDepartment of Bioenergy Science and Technology, Chonnam National University, Gwangju, 500-757, South Korea.
Takeshi FujinoDepartment of Bioenergy Science and Technology, Chonnam National University, Gwangju, 500-757, South Korea.
Sivakumar PattathilComplex Carbohydrate Research Center, University of Georgia, 31, Riverbend Road, Athens, GA, 30602, USA.
Chandrashekhar P JoshiDepartment of Bioenergy Science and Technology, Chonnam National University, Gwangju, 500-757, South Korea. cpjoshi@mtu.edu.
Chonnam National University · KRMichigan Technological University · USUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageFunctional characterization of two tobacco genes, one involved in xylan synthesis and the other, a positive regulator of secondary cell wall formation, is reported. Lignocellulosic secondary cell walls (SCW) provide essential plant materials for the production of second-generation bioethanol. Therefore, thorough understanding of the process of SCW formation in plants is beneficial for efficient bioethanol production. Recently, we provided the first proof-of-concept for using virus-induced gene silencing (VIGS) approach for rapid functional characterization of nine genes involved in cellulose, hemicellulose and lignin synthesis during SCW formation. Here, we report VIGS-mediated functional characterization of two tobacco genes involved in SCW formation. Stems of VIGS plants silenced for both selected genes showed increased amount of xylem formation but thinner cell walls than controls. These results were further confirmed by production of stable transgenic tobacco plants manipulated in expression of these genes. Stems of stable transgenic tobacco plants silenced for these two genes showed increased xylem proliferation with thinner walls, whereas transgenic tobacco plants overexpressing these two genes showed increased fiber cell wall thickness but no change in xylem proliferation. These two selected genes were later identified as possible members of DUF579 family involved in xylan synthesis and KNAT7 transcription factor family involved in positive regulation of SCW formation, respectively. Glycome analyses of cell walls showed increased polysaccharide extractability in 1 M KOH extracts of both VIGS-NbDUF579 and VIGS-NbKNAT7 lines suggestive of cell wall loosening. Also, VIGS-NbDUF579 and VIGS-NbKNAT7 lines showed increased saccharification rates (74.5 and 40 % higher than controls, respectively). All these properties are highly desirable for producing higher quantities of bioethanol from lignocellulosic materials of bioenergy plants.

Indexed as

Gene SilencingGenes, PlantCarbohydrate MetabolismCell WallFluorescein-5-isothiocyanateGene Expression Regulation, PlantGenetic VectorsGlucoseGlycomicsGlycosylationLigninNicotianaPlants, Genetically ModifiedPlant VirusesPolysaccharidesRNA, MessengerFluorescein-5-isothiocyanateGlucosehemicelluloseLigninlignocellulosePolysaccharidesRNA, MessengerBioethanol productionSaccharificationSecondary cell wall (SCW)Transcriptional regulationVirus-induced gene silencing (VIGS)Xylan synthesis

Identifiers

PMID27522520
OpenAlexW2516920513

What OpenQuestion holds

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Read underepoch 390

Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.