Evidence map›Paper›PMID 27521617›Full record

ArticleBiomaterials2016

Systematic optimization of an engineered hydrogel allows for selective control of human neural stem cell survival and differentiation after transplantation in the stroke brain.

Pouria Moshayedi, Lina R Nih, Irene L Llorente, Andrew R Berg, Jessica Cinkornpumin, William E Lowry, Tatiana Segura, S Thomas Carmichael

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 109 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
109citing papers in PubMed, 1 pooled it
11.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

109 citing papers in PubMed, 1 synthesis or guideline pooled it, 225 citations in OpenAlex.

  1. Pooled it
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  3. Review
  4. Piezo1 induces Wnt7bTheranostics · 2026
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  15. Cell reprogramming: methods, mechanisms and applications.Cell regeneration (London, England) · 2025
    Review
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  20. Article

49 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Pouria MoshayediDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 635 Charles Young Drive, CA 90095, USA.
Lina R NihDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, CA 90095, USA.
Irene L LlorenteDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 635 Charles Young Drive, CA 90095, USA.
Andrew R BergDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 635 Charles Young Drive, CA 90095, USA.
Jessica CinkornpuminDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, 710 Westwood Plaza, CA 90095, USA.
William E LowryDepartment of Molecular Cell and Developmental Biology, University of California, Los Angeles, 710 Westwood Plaza, CA 90095, USA.
Tatiana SeguraDepartment of Chemical and Biomolecular Engineering, University of California, Los Angeles, 420 Westwood Plaza, CA 90095, USA. Electronic address: tsegura@g.ucla.edu.
S Thomas CarmichaelDepartment of Neurology, David Geffen School of Medicine, University of California, Los Angeles, 635 Charles Young Drive, CA 90095, USA. Electronic address: scarmichael@mednet.ucla.edu.
University of California, Los Angeles · US

Funding

VEGF Ligand Presentation and Therapeutic AngiogenesisR01NS079691 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI SEGURA, TATIANA · 2012 to 2024
$4.0M
Stroke Stem Cell/Hydrogel TherapyR21NS067692 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CARMICHAEL, STANLEY THOMAS · 2009 to 2010
$421k
NINDS NIH HHS R01 NS079691NINDS NIH HHS R21 NS067692
6 · The paper itself

Abstract

Stem cell therapies have shown promise in promoting recovery in stroke but have been limited by poor cell survival and differentiation. We have developed a hyaluronic acid (HA)-based self-polymerizing hydrogel that serves as a platform for adhesion of structural motifs and a depot release for growth factors to promote transplant stem cell survival and differentiation. We took an iterative approach in optimizing the complex combination of mechanical, biochemical and biological properties of an HA cell scaffold. First, we optimized stiffness for a minimal reaction of adjacent brain to the transplant. Next hydrogel crosslinkers sensitive to matrix metalloproteinases (MMP) were incorporated as they promoted vascularization. Finally, candidate adhesion motifs and growth factors were systemically changed in vitro using a design of experiment approach to optimize stem cell survival or proliferation. The optimized HA hydrogel, tested in vivo, promoted survival of encapsulated human neural progenitor cells (iPS-NPCs) after transplantation into the stroke core and differentially tuned transplanted cell fate through the promotion of glial, neuronal or immature/progenitor states. This HA hydrogel can be tracked in vivo with MRI. A hydrogel can serve as a therapeutic adjunct in a stem cell therapy through selective control of stem cell survival and differentiation in vivo.

Indexed as

Tissue ScaffoldsAnimalsBrainCell DifferentiationCells, CulturedCell SurvivalGuided Tissue RegenerationHumansHyaluronic AcidHydrogelsMaleMaterials TestingMiceMice, Inbred C57BLNeural Stem CellsStem Cell TransplantationHyaluronic AcidHydrogelsAngiogenesisAstrocyteHyaluronaniPSNeural stem cellRegeneration

Identifiers

PMID27521617
PMCPMC5003628
OpenAlexW2506955896

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.