ArticleDevelopmental biology2016
Small RNA in situ hybridization in Caenorhabditis elegans, combined with RNA-seq, identifies germline-enriched microRNAs.
Article in Developmental biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 35 citations in OpenAlex.
- Optimal male fertility and fecundity in Caenorhabditis elegans require Microprocessor and Argonaute gene function.G3 (Bethesda, Md.) · 2026Article
- Exosome-mediated delivery of microRNAs by root-knot nematodes.PLoS pathogens · 2026Article
- A systems toxicology approach implicates post-transcriptional regulatory networks in reproductive defects from PFAS exposure.Toxicological sciences : an official journal of the Society of Toxicology · 2025Article
- The ribonuclease DISL-2 mediates piRNA degradation in C. elegans.Cell reports · 2025Article
- Widespread destabilization ofRNA (New York, N.Y.) · 2024Article
- Article
- Recent advances in understanding microRNA function and regulation in C. elegans.Seminars in cell & developmental biology · 2024Review
- Transcriptome-wide analyses of piRNA binding sites suggest distinct mechanisms regulate piRNA binding and silencing inRNA (New York, N.Y.) · 2023Article
- TRIM-NHL protein, NHL-2, modulates cell fate choices in the C. elegans germ line.Developmental biology · 2022Article
- Endogenous transcripts direct microRNA degradation in Drosophila, and this targeted degradation is required for proper embryonic development.Molecular cell · 2022Article
- Small RNAs couple embryonic developmental programs to gut microbes.Science advances · 2022Article
- Sperm fate is promoted by the mir-44 microRNA family in the Caenorhabditis elegans hermaphrodite germline.Genetics · 2021Article
- The multifaceted roles of microRNAs in differentiation.Current opinion in cell biology · 2020Review
- Kisspeptin Influences the Reproductive Axis and Circulating Levels of microRNAs in Senegalese Sole.International journal of molecular sciences · 2020Article
- Tissue- and sex-specific small RNAomes reveal sex differences in response to the environment.PLoS genetics · 2019Article
- Functional genomic analysis identifies miRNA repertoire regulating C. elegans oocyte development.Nature communications · 2018Article
- Recent Molecular Genetic Explorations ofGenetics · 2018Article
- C. elegans SUP-46, an HNRNPM family RNA-binding protein that prevents paternally-mediated epigenetic sterility.BMC biology · 2017Article
- Functional analysis of microRNA pathway genes in the somatic gonad and germ cells during ovulation in C. elegans.Developmental biology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Over four hundred different microRNAs (miRNAs) have been identified in the genome of the model organism the nematode Caenorhabditis elegans. As the germline is dedicated to the preservation of each species, and almost half of all the cells in an adult nematode are germline, it is likely that regulatory miRNAs are important for germline development and maintenance. In C. elegans the miR35 family has strong maternal effects, contributing to normal embryogenesis and to adult fecundity. To determine whether any particular miRNAs are greatly enriched in the C. elegans germline we used RNA-seq to compare the miRNA populations in several germline-defective strains of adult C. elegans worms, including glp-4(germline proliferation-4), glh-1(germline helicase-1) and dcr-1(dicer-1). Statistical analyses of RNA-seq comparisons identified 13 miRNAs that are germline-enriched, including seven members of the well-studied miR35 family that were reduced as much as 1000-fold in TaqMan qRT PCR miRNA assays. Along with the miR35s, six others: miR-56 (a member of the miR51 family),-70, -244, -260 , -788 and -4813, none of which previously considered as such, were also identified by RNA-seq as germline-enriched candidates. We went on to develop a successful miRNA in situ hybridization protocol for C. elegans, revealing miR35s specifically concentrate during oogenesis in the pachytene region of the gonad, and persist throughout early embryogenesis, while in adult animals neither let-7 nor miR-228 has a germline-bias.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.