ArticleFEMS microbiology letters2016
PCR-based detection of composite transposons and translocatable units from oral metagenomic DNA.
Article in FEMS microbiology letters, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- ISmLife · 2026Article
- PCR analysis of insertion sequences leads to the generation of artefact amplicons.Access microbiology · 2026Article
- Geographic signatures in the oral resistome: a comparative metagenomic analysis of healthy individuals from Thailand and Norway.Journal of oral microbiology · 2025Article
- Impact of cell lysis treatment before saliva metagenomic DNA extraction on the oral microbiome and the associated resistome.Clinical and experimental dental research · 2024Article
- Palidis: fast discovery of novel insertion sequences.Microbial genomics · 2023Article
- The first discovery of Tc1 transposons in yeast.Frontiers in microbiology · 2023Article
- Functional and Sequence-Specific Screening Protocols for the Detection of Novel Antimicrobial Resistance Genes in Metagenomic DNA.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Inverse PCR-based detection reveal novel mobile genetic elements and their associated genes in the human oral metagenome.BMC oral health · 2022Article
- Functional Metagenomic Screening for Antimicrobial Resistance in the Oral Microbiome.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Detection of mobile genetic elements associated with antibiotic resistance in Salmonella enterica using a newly developed web tool: MobileElementFinder.The Journal of antimicrobial chemotherapy · 2021Article
- Portable sequencer in the fight against infectious disease.Journal of human genetics · 2020Review
- Presence and Characterization of a NovelFrontiers in microbiology · 2020Article
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Authors and funding
3 authors.
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Abstract
A composite transposon is a mobile genetic element consisting of two insertion sequences (ISs) flanking a segment of cargo DNA often containing antibiotic resistance (AR) genes. Composite transposons can move as a discreet unit. There have been recently several reports on a novel mechanism of movement of an IS26-based composite transposon through the formation of a translocatable unit (TU), carrying the internal DNA segment of a composite transposon and one copy of a flanking IS. In this study, we determined the presence of composite transposons and TUs in human oral metagenomic DNA using PCR primers from common IS elements. Analysis of resulting amplicons showed four different IS1216 composite transposons and one IS257 composite transposon in our metagenomic sample. As our PCR strategy would also detect TUs, PCR was carried out to detect circular TUs predicted to originate from these composite transposons. We confirmed the presence of two novel TUs, one containing an experimentally proven antiseptic resistance gene and another containing a putative universal stress response protein (UspA) encoding gene. This is the first report of a PCR strategy to amplify the DNA segment on composite transposons and TUs in metagenomic DNA. This can be used to identify AR genes associated with a variety of mobile genetic elements from metagenomes.
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