Evidence map›Paper›PMID 27520728›Full record

ArticleNanomedicine : nanotechnology, biology, and medicine2017

Physicochemical characterization of ferumoxytol, heparin and protamine nanocomplexes for improved magnetic labeling of stem cells.

L Henry Bryant, Saejeong J Kim, Matthew Hobson, Blerta Milo, Zsofia I Kovacs, Neekita Jikaria, Bobbi K Lewis, Maria A Aronova, Alioscka A Sousa, Guofeng Zhang and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nanomedicine : nanotechnology, biology, and medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Article
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  9. Review
  10. Article
  11. Review
  12. Article
  13. Molecular Imaging in Nanotechnology and Theranostics.Molecular imaging and biology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

L Henry BryantLaboratory of Diagnostic Radiology Research, Radiology and Imaging Sciences, Clinical Center, Bethesda, MD, USA. Electronic address: HBryant@cc.nih.gov.
Saejeong J KimFrank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Matthew HobsonFrank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Blerta MiloFrank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Zsofia I KovacsFrank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Neekita JikariaFrank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Bobbi K LewisFrank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA.
Maria A AronovaLaboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Alioscka A SousaLaboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA; Department of Biochemistry, Federal University of Sao Paulo, Sao Paulo, Brazil.
Guofeng ZhangLaboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Richard D LeapmanLaboratory of Cellular Imaging and Macromolecular Biophysics, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA.
Joseph A FrankLaboratory of Diagnostic Radiology Research, Radiology and Imaging Sciences, Clinical Center, Bethesda, MD, USA; Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, MD, USA; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, MD, USA. Electronic address: jfrank@helix.nih.gov.

Funding

Electron MicroscopyZICEB000080 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI ZHANG, GUOFENG · 2011 to 2025
$8.2M
Electron microscopy of therapeutic and diagnostic nanoparticlesZIAEB000083 · NIBIB · NATIONAL INSTITUTE OF BIOMEDICAL IMAGING AND BIOENGINEERING · PI LEAPMAN, RICHARD · 2012 to 2019
$1.2M
Pre-clinical evaluation of Magnetically labeled Cells for Cellular MRIZIACL090007 · CLC · CLINICAL CENTER · PI FRANK, JOSEPH · 2009 to 2018
$0k
Core Research Services for Molecular Imaging and Imaging SciencesZICCL040013 · CLC · CLINICAL CENTER · PI FRANK, JOSEPH · 2009 to 2025
$0k
Intramural NIH HHS ZIA CL090007
6 · The paper itself

Abstract

Stem cell-based therapies have become a major focus in regenerative medicine and to treat diseases. A straightforward approach combining three drugs, heparin (H), protamine (P) with ferumoxytol (F) in the form of nanocomplexes (NCs) effectively labeled stem cells for cellular MRI. We report on the physicochemical characteristics for optimizing the H, P, and F components in different ratios, and mixing sequences, producing NCs that varied in hydrodynamic size. NC size depended on the order in which drugs were mixed in media. Electron microscopy of HPF or FHP showed that F was located on the surface of spheroidal shaped HP complexes. Human stem cells incubated with FHP NCs resulted in a significantly greater iron concentration per cell compared to that found in HPF NCs with the same concentration of F. These results indicate that FHP could be useful for labeling stem cells in translational studies in the clinic.

Indexed as

Ferrosoferric OxideHeparinProtaminesStem CellsCell TrackingHumansMagnetic Resonance ImagingMagneticsNanoparticlesStem Cell TransplantationFerrosoferric OxideHeparinProtaminesCell labelingFerumoxytolHeparinProtamineStem cells

Identifiers

PMID27520728
PMCPMC5298948

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.