Evidence map›Paper›PMID 27520549›Full record

ArticleJournal of industrial microbiology & biotechnology2016

Increased acid resistance of the archaeon, Metallosphaera sedula by adaptive laboratory evolution.

Chenbing Ai, Samuel McCarthy, Valerie Eckrich, Deepak Rudrappa, Guanzhou Qiu, Paul Blum

Open access · greenAbstract read
PubMed Publisher
In one paragraph

Article in Journal of industrial microbiology & biotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.6field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 35 citations in OpenAlex.

  1. Review
  2. Recent progress in adaptive laboratory evolution of industrial microorganisms.Journal of industrial microbiology & biotechnology · 2023
    Article
  3. Review
  4. Article
  5. Article
  6. Article
  7. Acid Experimental Evolution of the HaloarchaeonFrontiers in microbiology · 2020
    Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Biotechnology of extremely thermophilic archaea.FEMS microbiology reviews · 2018
    Review
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Chenbing AiSchool of Biological Sciences, University of Nebraska-Lincoln, E234 Beadle Center for Genetics, 1901 Vine St., Lincoln, NE, 68588-0666, USA.
Samuel McCarthySchool of Biological Sciences, University of Nebraska-Lincoln, E234 Beadle Center for Genetics, 1901 Vine St., Lincoln, NE, 68588-0666, USA.
Valerie EckrichSchool of Biological Sciences, University of Nebraska-Lincoln, E234 Beadle Center for Genetics, 1901 Vine St., Lincoln, NE, 68588-0666, USA.
Deepak RudrappaSchool of Biological Sciences, University of Nebraska-Lincoln, E234 Beadle Center for Genetics, 1901 Vine St., Lincoln, NE, 68588-0666, USA.
Guanzhou QiuSchool of Minerals Processing and Bioengineering, Central South University, Changsha, China.
Paul BlumSchool of Biological Sciences, University of Nebraska-Lincoln, E234 Beadle Center for Genetics, 1901 Vine St., Lincoln, NE, 68588-0666, USA. pblum1@unl.edu.
University of Nebraska–Lincoln · USCentral South University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Extremely thermoacidophilic members of the Archaea such as the lithoautotroph, Metallosphaera sedula, are among the most acid resistant forms of life and are of great relevance in bioleaching. Here, adaptive laboratory evolution was used to enhance the acid resistance of this organism while genomics and transcriptomics were used in an effort to understand the molecular basis for this trait. Unlike the parental strain, the evolved derivative, M. sedula SARC-M1, grew well at pH of 0.90. Enargite (Cu3AsS4) bioleaching conducted at pH 1.20 demonstrated SARC-M1 leached 23.78 % more copper relative to the parental strain. Genome re-sequencing identified two mutations in SARC-M1 including a nonsynonymous mutation in Msed_0408 (an amino acid permease) and a deletion in pseudogene Msed_1517. Transcriptomic studies by RNA-seq of wild type and evolved strains at various low pH values demonstrated there was enhanced expression of genes in M. sedula SARC-M1 encoding membrane complexes and enzymes that extrude protons or that catalyze proton-consuming reactions. In addition, M. sedula SARC-M1 exhibited reduced expression of genes encoding enzymes that catalyze proton-generating reactions. These unique genomic and transcriptomic features support a model for increased acid resistance arising from enhanced control over cytoplasmic pH.

Indexed as

Archaeal ProteinsCopperDirected Molecular EvolutionGene Expression ProfilingGenomicsHeterotrophic ProcessesHydrogen-Ion ConcentrationMutationSulfolobaceaeArchaeal ProteinsCopperAcidity resistanceEnargite bioleachingGenome re-sequencingMetallosphaera sedulaRNA sequencingTranscriptional responses

Identifiers

PMID27520549
OpenAlexW2512463681

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.