ArticleBiologicals : journal of the International Association of Biological Standardization2016
Evaluation of a microfluidic chip system for preparation of bacterial DNA from swabs, air, and surface water samples.
Article in Biologicals : journal of the International Association of Biological Standardization, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 10 citations in OpenAlex.
- A review of commercial and laboratory-based microfluidic devices based on glass and/or silicon substrates.RSC advances · 2026Review
- Recent Progress and Challenges on the Microfluidic Assay of Pathogenic Bacteria Using Biosensor Technology.Biomimetics (Basel, Switzerland) · 2022Review
- Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation.Microbial biotechnology · 2022Review
- Emission Sources of Campylobacter from Agricultural Farms, Impact on Environmental Contamination and Intervention Strategies.Current topics in microbiology and immunology · 2021Article
- Enhanced solid phase extraction of DNA using hydrophilic monodisperse poly(methacrylic acid-co-ethylene dimethacrylate) microparticles.Molecular biology reports · 2019Article
- Near real-time enumeration of live and dead bacteria using a fibre-based spectroscopic device.Scientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The detection of bacterial pathogens from complex sample matrices by PCR requires efficient DNA extraction. In this study, a protocol for extraction and purification of DNA from swabs, air, and water samples using a microfluidic chip system was established. The optimized protocol includes a combination of thermal, chemical and enzymatic lysis followed by chip-based DNA purification using magnetic particles. The procedure was tested using Gram-positive Bacillus thuringiensis Berliner var. kurstaki as a model organism for Bacillus anthracis and the attenuated live vaccine strain of Francisella tularensis subsp. holarctica as Gram-negative bacterium. The detection limits corresponded to 10
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.