ArticleBlood2016
RNA polymerase II pausing modulates hematopoietic stem cell emergence in zebrafish.
Article in Blood, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 37 citations in OpenAlex.
- Article
- Next-generation leukemia diagnostics: Integrating LC-MS/MS proteomics with liquid biopsy platforms.The journal of liquid biopsy · 2025Review
- Exploring novel protein biomarkers for early-stage diagnosis and prognosis of T-acute lymphoblastic leukemia (T-ALL).Hematology, transfusion and cell therapy · 2024Article
- Loss-of-Function Variants inInternational journal of molecular sciences · 2024Review
- RNA polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.Developmental cell · 2023Article
- RNA Polymerase II pausing temporally coordinates cell cycle progression and erythroid differentiation.medRxiv : the preprint server for health sciences · 2023Article
- Splicing factor deficits render hematopoietic stem and progenitor cells sensitive to STAT3 inhibition.Cell reports · 2022Article
- The hemoglobinopathies, molecular disease mechanisms and diagnostics.International journal of laboratory hematology · 2022Review
- RNA Pol II pausing facilitates phased pluripotency transitions by buffering transcription.Genes & development · 2022Article
- SETD5 modulates homeostasis of hematopoietic stem cells by mediating RNA Polymerase II pausing in cooperation with HCF-1.Leukemia · 2022Article
- Negative Elongation Factor (NELF) Inhibits Premature Granulocytic Development in Zebrafish.International journal of molecular sciences · 2022Article
- RNA polymerase II pausing in development: orchestrating transcription.Open biology · 2022Review
- The Paf1 complex and P-TEFb have reciprocal and antagonist roles in maintaining multipotent neural crest progenitors.Development (Cambridge, England) · 2019Article
- Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway.Cell death & disease · 2018Article
- Epigenetic and Epitranscriptomic Factors Make a Mark on Hematopoietic Stem Cell Development.Current stem cell reports · 2018Article
- Transcription pausing regulates mouse embryonic stem cell differentiation.Stem cell research · 2017Article
- Developmental HSC Microenvironments: Lessons from Zebrafish.Advances in experimental medicine and biology · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
The promoter-proximal pausing of RNA polymerase II (Pol II) plays a critical role in regulating metazoan gene transcription. Despite the prevalence of Pol II pausing across the metazoan genomes, little is known about the in vivo effect of Pol II pausing on vertebrate development. We use the emergence of hematopoietic stem cells (HSCs) in zebrafish embryos as a model to investigate the role of Pol II pausing in vertebrate organogenesis. Disrupting Pol II pausing machinery causes a severe reduction of HSC specification, a defect that can be effectively rescued by inhibiting Pol II elongation. In pausing-deficient embryos, the transforming growth factor β (TGFβ) signaling is elevated due to enhanced transcription elongation of key pathway genes, leading to HSC inhibition; in contrast, the interferon-γ (IFN-γ) signaling and its downstream effector Jak2/Stat3, which are required for HSC formation, are markedly attenuated owing to reduced chromatin accessibility on IFN-γ receptor genes. These findings reveal a novel transcription mechanism instructing HSC fate by pausing-mediated differential regulation of key signaling pathways.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.