ArticleBMC genomics2016
Regulatory complexity revealed by integrated cytological and RNA-seq analyses of meiotic substages in mouse spermatocytes.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed.
- Dissecting the development of bovine testicular tissue using spatial transcriptomics.Journal of animal science and biotechnology · 2026Article
- Unusual RNA polymerase II patterns in meiotic prophase I inTurkish journal of biology = Turk biyoloji dergisi · 2026Article
- Review
- Autophagy regulation and protein kinase activity of PIK3C3 controls sertoli cell polarity through its negative regulation on SCIN (scinderin).Autophagy · 2023Article
- An ancient testis-specific IQ motif-containing H gene regulates specific transcript isoform expression during spermatogenesis.Development (Cambridge, England) · 2023Article
- The First Analysis of Synaptonemal Complexes in Jawless Vertebrates: Chromosome Synapsis and Transcription Reactivation at Meiotic Prophase I in the LampreyLife (Basel, Switzerland) · 2023Article
- Decoding the Spermatogenesis Program: New Insights from Transcriptomic Analyses.Annual review of genetics · 2022Review
- Review
- YTHDC2 control of gametogenesis requires helicase activity but not mGenes & development · 2022Article
- Single-cell RNA-sequencing reveals the dynamic process and novel markers in porcine spermatogenesis.Journal of animal science and biotechnology · 2021Article
- Spermatogonial Gene Networks Selectively Couple to Glutathione and Pentose Phosphate Metabolism but Not Cysteine Biosynthesis.iScience · 2021Article
- The Application of Single-Cell RNA Sequencing in Mammalian Meiosis Studies.Frontiers in cell and developmental biology · 2021Review
- Transcriptomics of Meiosis in the Male Mouse.Frontiers in cell and developmental biology · 2021Review
- Regulation of meiotic progression by Sertoli-cell androgen signaling.Molecular biology of the cell · 2020Article
- HELLS and PRDM9 form a pioneer complex to open chromatin at meiotic recombination hot spots.Genes & development · 2020Article
- Genomic Structure ofGenetics · 2019Article
- A first genetic portrait of synaptonemal complex variation.PLoS genetics · 2019Article
- Mammalian SWI/SNF collaborates with a polycomb-associated protein to regulate male germline transcription in the mouse.Development (Cambridge, England) · 2019Article
- Hybrid Sterility with Meiotic Metaphase Arrest in Intersubspecific Mouse Crosses.The Journal of heredity · 2019Article
- Uncoupling of transcriptomic and cytological differentiation in mouse spermatocytes with impaired meiosis.Molecular biology of the cell · 2019Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
backgroundThe continuous and non-synchronous nature of postnatal male germ-cell development has impeded stage-specific resolution of molecular events of mammalian meiotic prophase in the testis. Here the juvenile onset of spermatogenesis in mice is analyzed by combining cytological and transcriptomic data in a novel computational analysis that allows decomposition of the transcriptional programs of spermatogonia and meiotic prophase substages.
resultsGerm cells from testes of individual mice were obtained at two-day intervals from 8 to 18 days post-partum (dpp), prepared as surface-spread chromatin and immunolabeled for meiotic stage-specific protein markers (STRA8, SYCP3, phosphorylated H2AFX, and HISTH1T). Eight stages were discriminated cytologically by combinatorial antibody labeling, and RNA-seq was performed on the same samples. Independent principal component analyses of cytological and transcriptomic data yielded similar patterns for both data types, providing strong evidence for substage-specific gene expression signatures. A novel permutation-based maximum covariance analysis (PMCA) was developed to map co-expressed transcripts to one or more of the eight meiotic prophase substages, thereby linking distinct molecular programs to cytologically defined cell states. Expression of meiosis-specific genes is not substage-limited, suggesting regulation of substage transitions at other levels.
conclusionsThis integrated analysis provides a general method for resolving complex cell populations. Here it revealed not only features of meiotic substage-specific gene expression, but also a network of substage-specific transcription factors and relationships to potential target genes.
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