ArticleInternational journal of molecular sciences2016
An Amphiprotic Novel Chitosanase from Bacillus mycoides and Its Application in the Production of Chitooligomers with Their Antioxidant and Anti-Inflammatory Evaluation.
Article in International journal of molecular sciences, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 69 citations in OpenAlex.
- Comparative Review of Microbial Chitosanases: Properties, Structures, Hydrolytic Modes and Applications.Current microbiology · 2026Review
- Effect of Chitooligosaccharides on TLR2/NF-κB Signaling in LPS-Stimulated RAW 264.7 Macrophages.Molecules (Basel, Switzerland) · 2025Article
- Analysis of chitinase gene family in barley and function study of HvChi22 involved in drought tolerance.Molecular biology reports · 2024Article
- Article
- The Discovery, Enzymatic Characterization and Functional Analysis of a Newly Isolated Chitinase from Marine-Derived FungusMarine drugs · 2022Article
- Biodegradation and Prospect of Polysaccharide from Crustaceans.Marine drugs · 2022Review
- Controllable preparation of chitosan oligosaccharidesFrontiers in microbiology · 2022Article
- Pilot-Scale Production of Chito-Oligosaccharides Using an Innovative Recombinant Chitosanase Preparation Approach.Polymers · 2021Article
- Cloning and Characterization of a New Chitosanase From a Deep-Sea BacteriumFrontiers in microbiology · 2021Article
- Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review.Molecules (Basel, Switzerland) · 2020Review
- Bioprocessing of Squid Pens Waste into Chitosanase byPolymers · 2020Article
- Coagulation of Chitin Production Wastewater from Shrimp Scraps with By-Product Chitosan and Chemical Coagulants.Polymers · 2020Article
- Novel Efficient Bioprocessing of Marine Chitins into Active Anticancer Prodigiosin.Marine drugs · 2019Article
- An Exochitinase withPolymers · 2019Article
- Reclamation of Fishery Processing Waste: A Mini-Review.Molecules (Basel, Switzerland) · 2019Article
- Production of a Thermostable Chitosanase from Shrimp Heads viaMarine drugs · 2019Article
- Chitooligosaccharides Prevents the Development of Colitis-Associated Colorectal Cancer by Modulating the Intestinal Microbiota and Mycobiota.Frontiers in microbiology · 2019Article
- Bioactivity-Guided Purification of Novel Herbal Antioxidant and Anti-NO Compounds fromMolecules (Basel, Switzerland) · 2018Article
- Reclamation of Marine Chitinous Materials for Chitosanase Production via Microbial Conversion byMarine drugs · 2018Article
- A Review of the Preparation, Analysis and Biological Functions of Chitooligosaccharide.International journal of molecular sciences · 2018Review
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objectives of this investigation were to produce a novel chitosanase for application in industries and waste treatment. The transformation of chitinous biowaste into valuable bioactive chitooligomers (COS) is one of the most exciting applications of chitosanase. An amphiprotic novel chitosanase from Bacillus mycoides TKU038 using squid pen powder (SPP)-containing medium was retrieved from a Taiwan soil sample, which was purified by column chromatography, and characterized by biochemical protocol. Extracellular chitosanase (CS038) was purified to 130-fold with a 35% yield, and its molecular mass was roughly 48 kDa. CS038 was stable over a wide range of pH values (4-10) at 50 °C and exhibited an optimal temperature of 50 °C. Interestingly, the optimum pH values were estimated as 6 and 10, whereas CS038 exhibited chitosan-degrading activity (100% and 94%, respectively). CS038 had Km and Vmax values of 0.098 mg/mL and 1.336 U/min, separately, using different concentrations of water-soluble chitosan. A combination of the high performance liquid chromatography (HPLC) and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometer data revealed that the chitosan oligosaccharides obtained from the hydrolysis of chitosan by CS038 comprise oligomers with multiple degrees of polymerization (DP), varying from 3-9, as well as CS038 in an endolytic fashion. The TKU038 culture supernatant and COS mixture exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activities. The COS activities were dose dependent and correlated to their DP. The COS with high DP exhibited enhanced DPPH radical scavenging capability compared with COS with low DP. Furthermore, the COS exhibited inhibitory behavior on nitric oxide (NO) production in murine RAW 264.7 macrophage cells, which was induced by Escherichia coli O111 lipopolysaccharide (LPS). The COS with low DP possesses a more potent anti-inflammatory capability to decrease NO production (IC50, 76.27 ± 1.49 µg/mL) than that of COS with high DP (IC50, 82.65 ± 1.18 µg/mL). Given its effectiveness in production and purification, acidophilic and alkalophilic properties, stability over ranges of pH values, ability to generate COS, antioxidant activity, and anti-inflammatory, CS038 has potential applications in SPP waste treatment and industries for COS production as a medical prebiotic.
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