ArticlePLoS genetics2016
Multiple Independent Retroelement Insertions in the Promoter of a Stress Response Gene Have Variable Molecular and Functional Effects in Drosophila.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 55 citations in OpenAlex.
- Retrotransposon activation during spermatogenesis achieves massive ecDNA biogenesis but rare integration.Genes & development · 2026Article
- Cold adaptation mechanism in Bactrocera dorsalis (Diptera: Tephritidae) by regulating MAPK signaling and metabolic pathways.Journal of insect science (Online) · 2026Article
- Article
- Toward Transgene-Free Transposon-Mediated Biological Mutagenesis for Plant Breeding.International journal of molecular sciences · 2023Review
- ChimeraTE: a pipeline to detect chimeric transcripts derived from genes and transposable elements.Nucleic acids research · 2023Article
- P-element invasion fuels molecular adaptation in laboratory populations of Drosophila melanogaster.Evolution; international journal of organic evolution · 2023Article
- The Interplay Between Developmental Stage and Environment Underlies the Adaptive Effect of a Natural Transposable Element Insertion.Molecular biology and evolution · 2023Article
- Gene expression differences consistent with water loss reduction underlie desiccation tolerance of natural Drosophila populations.BMC biology · 2023Article
- The genomic basis of copper tolerance in Drosophila is shaped by a complex interplay of regulatory and environmental factors.BMC biology · 2022Article
- Dynamics and Impacts of Transposable Element Proliferation in the Drosophila nasuta Species Group Radiation.Molecular biology and evolution · 2022Article
- Review
- Regulatory regions in natural transposable element insertions drive interindividual differences in response to immune challenges in Drosophila.Genome biology · 2021Article
- Transcription initiation of distant core promoters in a large-sized genome of an insect.BMC biology · 2021Article
- Genomic Analysis Revealed a Convergent Evolution of LINE-1 in Coat Color: A Case Study in Water Buffaloes (Bubalus bubalis).Molecular biology and evolution · 2021Article
- Insecticide exposure affects intergenerational patterns of DNA methylation in the Colorado potato beetle,Evolutionary applications · 2021Article
- Transposable elements contribute to the genomic response to insecticides inPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Article
- "What You Need, Baby, I Got It": Transposable Elements as Suppliers of Cis-Operating Sequences in Drosophila.Biology · 2020Review
- Population Genomics on the Fly: Recent Advances in Drosophila.Methods in molecular biology (Clifton, N.J.) · 2020Review
- Stress response, behavior, and development are shaped by transposable element-induced mutations in Drosophila.PLoS genetics · 2019Article
- A unique cluster ofMobile DNA · 2019Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Promoters are structurally and functionally diverse gene regulatory regions. The presence or absence of sequence motifs and the spacing between the motifs defines the properties of promoters. Recent alternative promoter usage analyses in Drosophila melanogaster revealed that transposable elements significantly contribute to promote diversity. In this work, we analyzed in detail one of the transposable element insertions, named FBti0019985, that has been co-opted to drive expression of CG18446, a candidate stress response gene. We analyzed strains from different natural populations and we found that besides FBti0019985, there are another eight independent transposable elements inserted in the proximal promoter region of CG18446. All nine insertions are solo-LTRs that belong to the roo family. We analyzed the sequence of the nine roo insertions and we investigated whether the different insertions were functionally equivalent by performing 5'-RACE, gene expression, and cold-stress survival experiments. We found that different insertions have different molecular and functional consequences. The exact position where the transposable elements are inserted matters, as they all showed highly conserved sequences but only two of the analyzed insertions provided alternative transcription start sites, and only the FBti0019985 insertion consistently affects CG18446 expression. The phenotypic consequences of the different insertions also vary: only FBti0019985 was associated with cold-stress tolerance. Interestingly, the only previous report of transposable elements inserting repeatedly and independently in a promoter region in D. melanogaster, were also located upstream of a stress response gene. Our results suggest that functional validation of individual structural variants is needed to resolve the complexity of insertion clusters.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.