ArticlePloS one2016
Molecular Characterization of Giardia lamblia: First Report of Assemblage B in Human Isolates from Rio de Janeiro (Brazil).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Genotypic and Epidemiologic Profiles of Giardia duodenalis in Four Brazilian Biogeographic RegionsMicroorganisms · 2022Article
- Comparative analysis of routine parasitological methods for recovery of cysts, molecular detection, and genotyping of Giardia duodenalis.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Prevalence of intestinal parasites with molecular detection and identification ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021Article
- Occurrence of Giardia duodenalis in selected stations and tributary rivers of Laguna Lake, Philippines.Environmental monitoring and assessment · 2021Article
- Occurrence of faecal endoparasites in reindeer (Rangifer tarandus) in two grazing areas in northern Norway.Acta veterinaria Scandinavica · 2021Article
- Multilocus Genotyping ofPathogens (Basel, Switzerland) · 2021Article
- Epidemiology of Giardia duodenalis assemblages in Brazil: there is still a long way to go.Memorias do Instituto Oswaldo Cruz · 2021Review
- The Predominance of Giardia duodenalis AII sub-assemblage in young children from Salvador, Bahia, Brazil.Biomedica : revista del Instituto Nacional de Salud · 2020Article
- Prevalence and genotypic characterization ofFood and waterborne parasitology · 2020Article
- Recirculation ofFrontiers in microbiology · 2020Article
- Prevalence of intestinal parasites, with emphasis on the molecular epidemiology of Giardia duodenalis and Blastocystis sp., in the Paranaguá Bay, Brazil: a community survey.Parasites & vectors · 2018Article
- Comparison ofTropical parasitologyArticle
- Molecular epidemiologic tools for waterborne pathogensFood and waterborne parasitologyReview
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5 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundDespite the high prevalence of giardiasis, the genetic characterization of Giardia lamblia has been poorly documented in Brazil and molecular epidemiology research has only been conducted in the last few years. The aim of this study was to determine the prevalence of different G. lamblia assemblages and detect mixed infections among patients with giardiasis. METHODS AND PRINCIPAL
findingsThe cross-section survey was conducted among patients attending the FIOCRUZ in Rio de Janeiro. In order to discriminate the genetic assemblages/sub-assemblages, G. lamblia isolates were characterized by PCR-RFLP and qPCR using four loci genes (bg, gdh, tpi and orfC4). Of the 65 positive samples, 41 (63.1%) were successfully amplified by nested-PCR of bg and gdh genes. Among them, 16 were typed as sub-assemblage AII, 7 as BIII, 4 as BIV and 8 as a mixture of BIII and BIV. After the analysis by qPCR assay, a total of 55 (84.6%) samples were amplified using at least one locus: bg gene was amplified in 38 (58.5%) samples, gdh in 41 (63.1%), tpi in 39 (60%), and orfC4 in 39 (60%). Multilocus genotyping results showed that 29 (52.7%) samples belonged to Assemblage A and 26 (47.3%) samples belonged to Assemblage B. In 2011 and 2012, 20 (74.1%) samples belonged to Assemblage A and 7 (25.9%) belonged to Assemblage B. In subsequent years (2013-2015) there was a predominance of Assemblage B, 19 (67.9%) versus 9 (32.1%) Assemblage A.
conclusionsThis is the first time that Assemblage B of G. lamblia was reported in human clinical samples from Rio de Janeiro (Brazil) and is the first report about genetic characterization using four genes. The qPCR assemblage-specific showed no mixed infections by Assemblages A and B. A switch in genetic profile over the years was observed, firstly predominance of Assemblage A and lastly of Assemblage B.
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