ArticleFrontiers in cellular neuroscience2016
Seizure-Induced Motility of Differentiated Dentate Granule Cells Is Prevented by the Central Reelin Fragment.
Article in Frontiers in cellular neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Inhibition of Granule Cell Dispersion and Seizure Development by Astrocyte Elevated Gene-1 in a Mouse Model of Temporal Lobe Epilepsy.Biomolecules · 2024Article
- Seizure-induced hilar ectopic granule cells in the adult dentate gyrus.Frontiers in neuroscience · 2023Review
- Hippocampal transplants of fetal GABAergic progenitors regulate adult neurogenesis in mice with temporal lobe epilepsy.Neurobiology of disease · 2022Article
- Proteomic differences in hippocampus and cortex of sudden unexplained death in childhood.Acta neuropathologica · 2022Article
- Tetrodotoxin prevents heat-shock induced granule cell dispersion in hippocampal slice cultures.Frontiers in cell and developmental biology · 2022Article
- Considering the Role of Extracellular Matrix Molecules, in Particular Reelin, in Granule Cell Dispersion Related to Temporal Lobe Epilepsy.Frontiers in cell and developmental biology · 2022Review
- Reelin Is Required for Maintenance of Granule Cell Lamination in the Healthy and Epileptic Hippocampus.Frontiers in molecular neuroscience · 2021Article
- Heat-Shock Induces Granule Cell Dispersion and Microgliosis in Hippocampal Slice Cultures.Frontiers in cell and developmental biology · 2021Article
- Reelin Functions, Mechanisms of Action and Signaling Pathways During Brain Development and Maturation.Biomolecules · 2020Review
- Hippocampal granule cell dispersion: a non-specific finding in pediatric patients with no history of seizures.Acta neuropathologica communications · 2020Article
- Selective Inactivation of Reelin in Inhibitory Interneurons Leads to Subtle Changes in the Dentate Gyrus But Leaves Cortical Layering and Behavior Unaffected.Cerebral cortex (New York, N.Y. : 1991) · 2020Article
- Granule Cell Dispersion in Human Temporal Lobe Epilepsy: Proteomics Investigation of Neurodevelopmental Migratory Pathways.Frontiers in cellular neuroscience · 2020Article
- Perspective: Therapeutic Potential of Flavonoids as Alternative Medicines in Epilepsy.Advances in nutrition (Bethesda, Md.) · 2019Article
- Article
- Drug repositioning in epilepsy reveals novel antiseizure candidates.Annals of clinical and translational neurology · 2019Article
- Prospects of Cannabidiol for Easing Status Epilepticus-Induced Epileptogenesis and Related Comorbidities.Molecular neurobiology · 2018Review
- Reelin: Neurodevelopmental Architect and Homeostatic Regulator of Excitatory Synapses.The Journal of biological chemistry · 2017Review
- Identification of Hub Genes of Mesio Temporal Lobe Epilepsy and Prognostic Biomarkers of Brain Low-grade Gliomas Based on Bioinformatics Analysis.Cell transplantationArticle
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Granule cell dispersion (GCD) represents a pathological widening of the granule cell layer in the dentate gyrus and it is frequently observed in patients with mesial temporal lobe epilepsy (MTLE). Recent studies in human MTLE specimens and in animal epilepsy models have shown that a decreased expression and functional inactivation of the extracellular matrix protein Reelin correlates with GCD formation, but causal evidence is still lacking. Here, we used unilateral kainate (KA) injection into the mouse hippocampus, an established MTLE animal model, to precisely map the loss of reelin mRNA-synthesizing neurons in relation to GCD along the septotemporal axis of the epileptic hippocampus. We show that reelin mRNA-producing neurons are mainly lost in the hilus and that this loss precisely correlates with the occurrence of GCD. To monitor GCD formation in real time, we used organotypic hippocampal slice cultures (OHSCs) prepared from mice which express enhanced green fluorescent protein (eGFP) primarily in differentiated dentate granule cells. Using life cell microscopy we observed that increasing doses of KA resulted in an enhanced motility of eGFP-positive granule cells. Moreover, KA treatment of OHSC resulted in a rapid loss of Reelin-producing interneurons mainly in the hilus, as observed in vivo. A detailed analysis of the migration behavior of individual eGFP-positive granule cells revealed that the majority of these neurons actively migrate toward the hilar region, where Reelin-producing neurons are lost. Treatment with KA and subsequent addition of the recombinant R3-6 Reelin fragment significantly prevented the movement of eGFP-positive granule cells. Together, these findings suggest that GCD formation is indeed triggered by a loss of Reelin in hilar interneurons.
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