ArticleGenome research2016
Critical roles of long noncoding RNAs in Drosophila spermatogenesis.
Article in Genome research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 92 papers.
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Who cites it
92 citing papers in PubMed, 204 citations in OpenAlex.
- Elevated LncRNAFASEB bioAdvances · 2026Article
- Functional analysis of lncRNAs regulating the TOR signaling pathway and reproduction in the white-backed planthopper, Sogatella furcifera.Pest management science · 2026Article
- Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive in Drosophila.Nature communications · 2026Article
- Defective transcription of AAGAG satellite DNA causes sex-ratio meiotic drive inbioRxiv : the preprint server for biology · 2026Article
- The landscape and evolutionary dynamics of derived lncRNAs in insects.Communications biology · 2025Article
- Male sterility in Drosophila mojavensis × D. arizonae hybrids is not caused by gene movements in the Y chromosome.Evolution; international journal of organic evolution · 2025Article
- Cell type- and chromosome-specific chromatin landscapes and DNA replication programs ofbioRxiv : the preprint server for biology · 2025Article
- Meiotic Sex Chromosome Inactivation: Conservation across the Drosophila genus.PLoS genetics · 2025Article
- lncRNAInternational journal of molecular sciences · 2025Article
- A polycistronic locus encodes both a tumor-suppressive microRNA and a long non-coding RNA regulating testis morphology in Drosophila.bioRxiv : the preprint server for biology · 2025Article
- Genomics of a sexually selected sperm ornament and female preference in Drosophila.Nature ecology & evolution · 2025Article
- Human sperm RNA in male infertility.Nature reviews. Urology · 2025Review
- Diversification and recurrent adaptation of the synaptonemal complex in Drosophila.PLoS genetics · 2025Article
- Identification of crucial LncRNAs associated with testicular development and LOC108635509 as a potential regulator in black goat spermatogenesis.BMC genomics · 2024Article
- Thousands of oscillating LncRNAs in the mouse testis.Computational and structural biotechnology journal · 2024Article
- Long noncoding RNAScience advances · 2024Article
- Loss-of-function in testis-specific serine/threonine protein kinase triggers male infertility in an invasive moth.Communications biology · 2024Article
- Core genes of biomineralization and cis-regulatory long non-coding RNA regulate shell growth in bivalves.Journal of advanced research · 2024Article
- A Synergistic, Cultivator Model of De Novo Gene Origination.Genome biology and evolution · 2024Article
- Regulation of insect behavior by non-coding RNAs.Science China. Life sciences · 2024Review
32 more citing papers are in PubMed but not listed here.
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Authors and funding
16 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Long noncoding RNAs (lncRNAs), a recently discovered class of cellular RNAs, play important roles in the regulation of many cellular developmental processes. Although lncRNAs have been systematically identified in various systems, most of them have not been functionally characterized in vivo in animal models. In this study, we identified 128 testis-specific Drosophila lncRNAs and knocked out 105 of them using an optimized three-component CRISPR/Cas9 system. Among the lncRNA knockouts, 33 (31%) exhibited a partial or complete loss of male fertility, accompanied by visual developmental defects in late spermatogenesis. In addition, six knockouts were fully or partially rescued by transgenes in a trans configuration, indicating that those lncRNAs primarily work in trans Furthermore, gene expression profiles for five lncRNA mutants revealed that testis-specific lncRNAs regulate global gene expression, orchestrating late male germ cell differentiation. Compared with coding genes, the testis-specific lncRNAs evolved much faster. Moreover, lncRNAs of greater functional importance exhibited higher sequence conservation, suggesting that they are under constant evolutionary selection. Collectively, our results reveal critical functions of rapidly evolving testis-specific lncRNAs in late Drosophila spermatogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.