ArticleProceedings of the National Academy of Sciences of the United States of America2016
FliW antagonizes CsrA RNA binding by a noncompetitive allosteric mechanism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
27 citing papers in PubMed, 39 citations in OpenAlex.
- Functional analysis of natural variation in the RNA-binding protein CsrA across the bacterial domain predicts regulatory activity.Nucleic acids research · 2026Article
- The last bacterial common ancestor encoded a complex flagellum.bioRxiv : the preprint server for biology · 2026Article
- FliW regulates biofilm formation in Geobacter sulfurreducens through interaction with CsrA.Applied microbiology and biotechnology · 2026Article
- The Flagellin-Specific Chaperone FliS of Borrelia burgdorferi Controls the Cytoplasmic Pool of Flagellins at the Level of Translation Initiation, Secretion, and Proteolysis.Molecular microbiology · 2025Article
- Swarm-seq Reveals Functional Divergence in CsrA Homologs Across Bacteria.bioRxiv : the preprint server for biology · 2025Article
- Control ofmBio · 2025Article
- The role of CsrA in controls the extracellular electron transfer and biofilm production inFrontiers in microbiology · 2025Article
- FlaG competes with FliS-flagellin complexes for access to FlhA in the flagellar T3SS to controlProceedings of the National Academy of Sciences of the United States of America · 2024Article
- Small proteins in Gram-positive bacteria.FEMS microbiology reviews · 2023Article
- E. coli Toxin YjjJ (HipH) Is a Ser/Thr Protein Kinase That Impacts Cell Division, Carbon Metabolism, and Ribosome Assembly.mSystems · 2023Article
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- The World of Stable Ribonucleoproteins and Its Mapping With Grad-Seq and Related Approaches.Frontiers in molecular biosciences · 2021Review
- FliW and CsrA Govern Flagellin (FliC) Synthesis and Play Pleiotropic Roles in Virulence and Physiology ofFrontiers in microbiology · 2021Article
- Contact with the CsrA Core Is Required for Allosteric Inhibition by FliW in Bacillus subtilis.Journal of bacteriology · 2020Article
- Comparative Genomics and Evolutionary Analysis of RNA-Binding Proteins of the CsrA Family in the GenusFrontiers in molecular biosciences · 2020Article
- Diverse Mechanisms and Circuitry for Global Regulation by the RNA-Binding Protein CsrA.Frontiers in microbiology · 2020Review
- Binding ofFrontiers in microbiology · 2020Article
- Intermolecular Communication inFrontiers in molecular biosciences · 2020Review
- Posttranscription Initiation Control of Gene Expression Mediated by Bacterial RNA-Binding Proteins.Annual review of microbiology · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
CsrA (carbon storage regulator A) is a widely distributed bacterial RNA binding protein that regulates translation initiation and mRNA stability of target transcripts. In γ-proteobacteria, CsrA activity is competitively antagonized by one or more small RNAs (sRNAs) containing multiple CsrA binding sites, but CsrA in bacteria outside the γ-proteobacteria is antagonized by a protein called FliW. Here we show that FliW of Bacillus subtilis does not bind to the same residues of CsrA required for RNA binding. Instead, CsrA mutants resistant to FliW antagonism (crw) altered residues of CsrA on an allosteric surface of previously unattributed function. Some crw mutants abolished CsrA-FliW binding, but others did not, suggesting that FliW and RNA interaction is not mutually exclusive. We conclude that FliW inhibits CsrA by a noncompetitive mechanism that differs dramatically from the well-established sRNA inhibitors. FliW is highly conserved with CsrA in bacteria, appears to be the ancestral form of CsrA regulation, and represents a widespread noncompetitive mechanism of CsrA control.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.