ArticlePlant physiology2016
Exocyst SEC3 and Phosphoinositides Define Sites of Exocytosis in Pollen Tube Initiation and Growth.
Article in Plant physiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 101 citations in OpenAlex.
- Molecular choreography of vesicle trafficking: Exocyst-SNARE interplay in plant defense.Journal of advanced research · 2026Review
- Heat stress-induced remodelling of lipid and microRNA networks in Brassica napus germinated pollen and pollen-derived small extracellular vesicles.Journal of experimental botany · 2026Article
- Article
- Reciprocal regulation between AtFH5-labeled secretory vesicles and PI(4,5)PJournal of integrative plant biology · 2025Article
- Genome-wide characterization and expression profiling of MADS-box family genes during organ development and drought stress in Camelina sativa L.Scientific reports · 2025Article
- A land plant-specific VPS13 mediates polarized vesicle trafficking in germinating pollen.The New phytologist · 2025Article
- Guidelines for naming and studying plasma membrane domains in plants.Nature plants · 2024Review
- Ectopic Expression of Distinct PLC Genes Identifies 'Compactness' as a Possible Architectural Shoot Strategy to Cope with Drought Stress.Plant & cell physiology · 2024Article
- RHO OF PLANTS signalling and the activating ROP GUANINE NUCLEOTIDE EXCHANGE FACTORS: specificity in cellular signal transduction in plants.Journal of experimental botany · 2024Review
- Phosphoinositides in plant-pathogen interaction: trends and perspectives.Stress biology · 2023Review
- Article
- Article
- Rab GTPases, tethers, and SNAREs work together to regulateFrontiers in plant science · 2023Review
- The premetazoan ancestry of the synaptic toolkit and appearance of first neurons.Essays in biochemistry · 2022Article
- The PH Domain and C-Terminal polyD Motif of Phafin2 Exhibit a Unique Concurrence in Animals.Membranes · 2022Article
- Let's shape again: the concerted molecular action that builds the pollen tube.Plant reproduction · 2022Review
- SEC1A is a major Arabidopsis Sec1/Munc18 gene in vesicle trafficking during pollen tube tip growth.The Plant journal : for cell and molecular biology · 2022Article
- A nanodomain-anchored scaffolding complex is required for the function and localization of phosphatidylinositol 4-kinase alpha in plants.The Plant cell · 2022Article
- Comparative Proteomic Analysis of Plasma Membrane Proteins in Rice Leaves Reveals a Vesicle Trafficking Network in Plant Immunity That Is Provoked by Blast Fungi.Frontiers in plant science · 2022Article
- Plasma membrane phospholipid signature recruits the plant exocyst complex via the EXO70A1 subunit.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polarized exocytosis is critical for pollen tube growth, but its localization and function are still under debate. The exocyst vesicle-tethering complex functions in polarized exocytosis. Here, we show that a sec3a exocyst subunit null mutant cannot be transmitted through the male gametophyte due to a defect in pollen tube growth. The green fluorescent protein (GFP)-SEC3a fusion protein is functional and accumulates at or proximal to the pollen tube tip plasma membrane. Partial complementation of sec3a resulted in the development of pollen with multiple tips, indicating that SEC3 is required to determine the site of pollen germination pore formation. Time-lapse imaging demonstrated that SEC3a and SEC8 were highly dynamic and that SEC3a localization on the apical plasma membrane predicts the direction of growth. At the tip, polar SEC3a domains coincided with cell wall deposition. Labeling of GFP-SEC3a-expressing pollen with the endocytic marker FM4-64 revealed the presence of subdomains on the apical membrane characterized by extensive exocytosis. In steady-state growing tobacco (Nicotiana tabacum) pollen tubes, SEC3a displayed amino-terminal Pleckstrin homology-like domain (SEC3a-N)-dependent subapical membrane localization. In agreement, SEC3a-N interacted with phosphoinositides in vitro and colocalized with a phosphatidylinositol 4,5-bisphosphate (PIP
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