ArticleDental materials : official publication of the Academy of Dental Materials2016
Real-time assessment of Streptococcus mutans biofilm metabolism on resin composite.
Article in Dental materials : official publication of the Academy of Dental Materials, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Antibacterial activity of a bioactive composite resins containing surface pre-reacted glass in a complex multispecies subgingival biofilm.Odontology · 2026Article
- Smart biomaterials in restorative dentistry: Recent advances and future perspectives.Materials today. Bio · 2025Review
- Optimization of an ultra-bright real-time high-throughput renilla luciferase assay for antibacterial assessment of Streptococcus mutans biofilms.Dental materials : official publication of the Academy of Dental Materials · 2024Article
- Antibacterial Activity of a Bioactive Tooth-Coating Material Containing Surface Pre-Reacted Glass in a Complex Multispecies Subgingival Biofilm.Pharmaceutics · 2023Article
- Shear Bond Strength and Color Stability of Novel Antibacterial Nanofilled Dental Adhesive Resins.Nanomaterials (Basel, Switzerland) · 2022Article
- Effect of brushing on surface roughness, fluoride release, and biofilm formation with different tooth-colored materials.Journal of dental sciences · 2022Article
- Antibacterial efficacy of non-thermal atmospheric plasma against Streptococcus mutans biofilm grown on the surfaces of restorative resin composites.Scientific reports · 2021Article
- Assessment of Streptococcus mutans biofilms on orthodontic adhesives over 7 days.American journal of orthodontics and dentofacial orthopedics : official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics · 2021Article
- High Counts and Anthracene Degradation Ability ofFrontiers in microbiology · 2021Article
- Ageing of Dental Composites Based on Methacrylate Resins-A Critical Review of the Causes and Method of Assessment.Polymers · 2020Review
- Optimization of a real-time high-throughput assay for assessment of Streptococcus mutans metabolism and screening of antibacterial dental adhesives.Dental materials : official publication of the Academy of Dental Materials · 2020Article
- Physicochemical, Mechanical, and Antimicrobial Properties of Novel Dental Polymers Containing Quaternary Ammonium and Trimethoxysilyl Functionalities.Journal of functional biomaterials · 2019Article
- Antibacterial dental adhesive resins containing nitrogen-doped titanium dioxide nanoparticles.Materials science & engineering. C, Materials for biological applications · 2018Article
- Evaluation of biofilm formation on novel copper-catalyzed azide-alkyne cycloaddition (CuAAC)-based resins for dental restoratives.Dental materials : official publication of the Academy of Dental Materials · 2018Article
- Quantitative and Qualitative Assessment Methods for Biofilm Growth: A Mini-review.Research & reviews. Journal of engineering and technology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveThe release of unpolymerized monomers and by-products of resin composites influences biofilm growth and confounds the measurement of metabolic activity. Current assays to measure biofilm viability have critical limitations and are typically not performed on relevant substrates. The objective of the present study was to determine the utility of firefly luciferase assay for quantification of the viability of intact biofilms on a resin composite substrate, and correlate the results with a standard method (viable colony counts).
methodsDisk-shaped specimens of a dental resin composite were fabricated, wet-polished, UV-sterilized, and stored in water. Biofilms of Streptococcus mutans (strain UA159 modified by insertion of constitutively expressed firefly luc gene) were grown (1:500 dilution; anaerobic conditions, 24h, 37°C) in two media concentrations (0.35x and 0.65x THY medium supplemented with 0.1% sucrose; n=15/group). An additional group of specimens with biofilms grown in 0.65x+sucrose media was treated with chlorhexidine gluconate solution to serve as the control group. Bioluminescence measurements of non-disrupted biofilms were obtained after addition of d-Luciferin substrate. The adherent biofilms were removed by sonication, and bioluminescence of sonicated bacteria was then measured. Viable colony counts were performed after plating sonicated bacteria on THY agar plates supplemented with spectinomycin. Bioluminescence values and cell counts were correlated using Spearman correlation tests (α=0.05).
resultsStrong positive correlations between viable colony counts and bioluminescence values, both before- and after-sonication, validated the utility of this assay. SIGNIFICANCE: A novel non-disruptive, real-time bioluminescence assay is presented for quantification of intact S. mutans biofilms grown on a resin composite, and potentially on antibacterial materials and other types of dental biomaterials.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.