Evidence map›Paper›PMID 27515427›Full record

ArticleStem cell research & therapy2016

Exendin-4 enhances the differentiation of Wharton's jelly mesenchymal stem cells into insulin-producing cells through activation of various β-cell markers.

Dina H Kassem, Mohamed M Kamal, Abd El-Latif G El-Kholy, Hala O El-Mesallamy

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell research & therapy, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 2 pooled it
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 2 syntheses or guidelines pooled it, 46 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Dina H KassemBiochemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Mohamed M KamalBiochemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Abd El-Latif G El-KholyGynecology and Obstetrics Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt.
Hala O El-MesallamyBiochemistry Department, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt. hala.elmesallamy@hotmail.com.
Ain Shams University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundDiabetes mellitus is a devastating metabolic disease. Generation of insulin-producing cells (IPCs) from stem cells, especially from Wharton's jelly mesenchymal stem cells (WJ-MSCs), has sparked much interest recently. Exendin-4 has several beneficial effects on MSCs and β cells. However, its effects on generation of IPCs from WJ-MSCs specifically have not been studied adequately. The purpose of this study was therefore to investigate how exendin-4 could affect the differentiation outcome of WJ-MSCs into IPCs, and to investigate the role played by exendin-4 in this differentiation process.

methodsWJ-MSCs were isolated, characterized and then induced to differentiate into IPCs using two differentiation protocols: protocol A, without exendin-4; and protocol B, with exendin-4. Differentiated IPCs were assessed by the expression of various β-cell-related markers using quantitative RT-PCR, and functionally by measuring glucose-stimulated insulin secretion.

resultsThe differentiation protocol B incorporating exendin-4 significantly boosted the expression levels of β-cell-related genes Pdx-1, Nkx2.2, Isl-1 and MafA. Moreover, IPCs generated by protocol B showed much better response to variable glucose concentrations as compared with those derived from protocol A, which totally lacked such response. Furthermore, exendin-4 alone induced early differentiation markers such as Pdx-1 and Nkx2.2 but not Isl-1, besides inducing late markers such as MafA. In addition, exendin-4 showed a synergistic effect with nicotinamide and β-mercaptoethanol in the induction of these markers.

conclusionsExendin-4 profoundly improves the differentiation outcome of WJ-MSCs into IPCs, possibly through the ability to induce the expression of β-cell markers.

Indexed as

BiomarkersCell DifferentiationCells, CulturedExenatideGlucoseHomeobox Protein Nkx-2.2Homeodomain ProteinsHumansInsulinInsulin-Secreting CellsMercaptoethanolMesenchymal Stem CellsNiacinamideNuclear ProteinsPeptidesStem CellsBiomarkersExenatideGlucoseHomeobox Protein Nkx-2.2Homeodomain ProteinsInsulinMercaptoethanolNiacinamideNKX2-2 protein, humanNuclear ProteinsPeptidesTranscription FactorsVenomsDiabetes mellitusExendin-4Insulin-producing cellsMesenchymal stem cellsWharton’s jelly

Identifiers

PMID27515427
PMCPMC4981957
OpenAlexW2507369178

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.