ArticleScientific reports2016
Globin mRNA reduction for whole-blood transcriptome sequencing.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 45 citations in OpenAlex.
- Integrated analysis of methylome and transcriptome responses to exercise training in children with overweight/obesity.Physiological genomics · 2025Trial
- Evaluation of Transcriptomic Regulations behind Metabolic Syndrome in Obese and Lean Subjects.International journal of molecular sciences · 2020Trial
- Technical Considerations for Blood RNA Sequencing in Genetic Testing: Evaluation of Globin Depletion Methods, Batch Effects, and Sample Types.Annals of laboratory medicine · 2026Article
- Role of oxysterol 4β-hydroxycholesterol and liver X receptor alleles in pre-eclampsia.Annals of medicine · 2025Article
- Signs of immune dysregulation in second-trimester maternal blood RNA profiles in late-onset preeclampsia.Scientific reports · 2025Article
- Biochemical-free enrichment or depletion of RNA classes in real-time during direct RNA sequencing with RISER.Nature communications · 2024Article
- Transcript capture and ultradeep long-read RNA sequencing (CAPLRseq) to diagnose HNPCC/Lynch syndrome.Journal of medical genetics · 2023Article
- Article
- Achieving symptom relief in patients with myalgic encephalomyelitis by targeting the neuro-immune interface and optimizing disease tolerance.Oxford open immunology · 2023Article
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- Robust Virus-Specific Adaptive Immunity in COVID-19 Patients with SARS-CoV-2 Δ382 Variant Infection.Journal of clinical immunology · 2022Article
- Generation of RNA sequencing libraries for transcriptome analysis of globin-rich tissues of the domestic dog.STAR protocols · 2021Article
- Cardiorespiratory fitness in children with overweight/obesity: Insights into the molecular mechanisms.Scandinavian journal of medicine & science in sports · 2021Article
- Dog colour patterns explained by modular promoters of ancient canid origin.Nature ecology & evolution · 2021Article
- Asymptomatic COVID-19: disease tolerance with efficient anti-viral immunity against SARS-CoV-2.EMBO molecular medicine · 2021Article
- Distinct whole-blood transcriptome profile of children with metabolic healthy overweight/obesity compared to metabolic unhealthy overweight/obesity.Pediatric research · 2021Article
- Peripheral blood transcriptomic sub-phenotypes of pediatric acute respiratory distress syndrome.Critical care (London, England) · 2020Observational
- Article
- A putative silencer variant in a spontaneous canine model of retinitis pigmentosa.PLoS genetics · 2020Article
- Methodologies for Transcript Profiling Using Long-Read Technologies.Frontiers in genetics · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 6 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transcriptome analysis of whole-blood RNA by sequencing holds promise for the identification and tracking of biomarkers; however, the high globin mRNA (gmRNA) content of erythrocytes hampers whole-blood and buffy coat analyses. We introduce a novel gmRNA locking assay (GlobinLock, GL) as a robust and simple gmRNA reduction tool to preserve RNA quality, save time and cost. GL consists of a pair of gmRNA-specific oligonucleotides in RNA initial denaturation buffer that is effective immediately after RNA denaturation and adds only ten minutes of incubation to the whole cDNA synthesis procedure when compared to non-blood RNA analysis. We show that GL is fully effective not only for human samples but also for mouse and rat, and so far incompletely studied cow, dog and zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.