Evidence map›Paper›PMID 27514990›Full record

ArticleScientific reports2016

Neuroprotective effects of apigenin against inflammation, neuronal excitability and apoptosis in an induced pluripotent stem cell model of Alzheimer's disease.

Rachelle Balez, Nicole Steiner, Martin Engel, Sonia Sanz Muñoz, Jeremy Stephen Lum, Yizhen Wu, Dadong Wang, Pascal Vallotton, Perminder Sachdev, Michael O'Connor and 3 more

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06560216 (Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm), which is not on this map. Cited by 131 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
131citing papers in PubMed, 3 pooled it
12.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06560216 nacompletednot on this mapstarted 2024, after this paper: background citation

Effects of Supplementation of Cryopreservation Medium With Apigenin on Post-thaw Human Sperm

TypeinterventionalSponsorChulalongkorn UniversityRan2024 to 2025Enrolled57ConditionsHealthyArmsApigenin
3 · Its place in the literature

Who cites it

131 citing papers in PubMed, 3 syntheses or guidelines pooled it, 277 citations in OpenAlex.

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  17. Stem Cell Interventions in the Treatment of Alzheimer's Disease.Current stem cell research & therapy · 2025
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71 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 1 country.

Rachelle BalezIllawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia.
Nicole SteinerSchool of Medicine, Western Sydney University, Locked bag 1797, Penrith, NSW, Australia.
Martin EngelIllawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia.
Sonia Sanz MuñozIllawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia.
Jeremy Stephen LumIllawarra Health and Medical Research Institute, School of Medicine, University of Wollongong, Wollongong, NSW, 2522, Australia.
Yizhen WuIllawarra Health and Medical Research Institute, School of Medicine, University of Wollongong, Wollongong, NSW, 2522, Australia.
Dadong WangCSIRO Informatics and Statistics, Locked Bag 17, North Ryde, NSW 1670, Australia.
Pascal VallottonCSIRO Informatics and Statistics, Locked Bag 17, North Ryde, NSW 1670, Australia.
Perminder SachdevCentre for Healthy Brain Ageing School of Medicine, University of New South Wales, High Street, Kensington,. NSW, 2052, Australia.
Michael O'ConnorSchool of Medicine, Western Sydney University, Locked bag 1797, Penrith, NSW, Australia.
Kuldip SidhuCentre for Healthy Brain Ageing School of Medicine, University of New South Wales, High Street, Kensington,. NSW, 2052, Australia.
Gerald MünchSchool of Medicine, Western Sydney University, Locked bag 1797, Penrith, NSW, Australia.
Lezanne OoiIllawarra Health and Medical Research Institute, School of Biological Sciences, University of Wollongong, Wollongong, NSW, 2522, Australia.
Illawarra Health and Medical Research Institute · AUUniversity of Wollongong · AUWestern Sydney University · AUCommonwealth Scientific and Industrial Research Organisation · AUUNSW Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Alzheimer's disease (AD) is one of the most prevalent neurodegenerative diseases, yet current therapeutic treatments are inadequate due to a complex disease pathogenesis. The plant polyphenol apigenin has been shown to have anti-inflammatory and neuroprotective properties in a number of cell and animal models; however a comprehensive assessment has not been performed in a human model of AD. Here we have used a human induced pluripotent stem cell (iPSC) model of familial and sporadic AD, in addition to healthy controls, to assess the neuroprotective activity of apigenin. The iPSC-derived AD neurons demonstrated a hyper-excitable calcium signalling phenotype, elevated levels of nitrite, increased cytotoxicity and apoptosis, reduced neurite length and increased susceptibility to inflammatory stress challenge from activated murine microglia, in comparison to control neurons. We identified that apigenin has potent anti-inflammatory properties with the ability to protect neurites and cell viability by promoting a global down-regulation of cytokine and nitric oxide (NO) release in inflammatory cells. In addition, we show that apigenin is able to protect iPSC-derived AD neurons via multiple means by reducing the frequency of spontaneous Ca(2+) signals and significantly reducing caspase-3/7 mediated apoptosis. These data demonstrate the broad neuroprotective action of apigenin against AD pathogenesis in a human disease model.

Indexed as

AdultAgedAlzheimer DiseaseAnimalsApigeninApoptosisCalcium SignalingCase-Control StudiesCell DifferentiationCells, CulturedCytokinesDown-RegulationHumansInduced Pluripotent Stem CellsInflammationInterferon-gammaApigeninCytokinesInterferon-gammaLipopolysaccharidesNeuroprotective Agents

Identifiers

PMID27514990
PMCPMC4981845
OpenAlexW2515262002

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.