ArticleMethods in molecular biology (Clifton, N.J.)2016
Methods for Studying Movement of Molecules Within Cilia.
Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- PKD2-like proteins targetProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Importin-9 and a TPR domain protein MpH drive periodic patterning of ciliary arrays in Tetrahymena.The Journal of cell biology · 2025Article
- Distribution and bulk flow analyses of the intraflagellar transport (IFT) motor kinesin-2 support an "on-demand" model for Chlamydomonas ciliary length control.Cytoskeleton (Hoboken, N.J.) · 2024Article
- Global and local functions of the Fused kinase ortholog CdaH in intracellular patterning in Tetrahymena.Journal of cell science · 2024Article
- The Small Interactor of PKD2 protein promotes the assembly and ciliary entry of the Chlamydomonas PKD2-mastigoneme complexes.Journal of cell science · 2024Article
- In vivo imaging reveals independent intraflagellar transport of the nexin-dynein regulatory complex subunits DRC2 and DRC4.Molecular biology of the cell · 2023Article
- Wbm0076, a candidate effector protein of the Wolbachia endosymbiont of Brugia malayi, disrupts eukaryotic actin dynamics.PLoS pathogens · 2023Article
- Loss of ARL13 impedes BBSome-dependent cargo export from Chlamydomonas cilia.The Journal of cell biology · 2022Article
- Article
- In vivo imaging shows continued association of several IFT-A, IFT-B and dynein complexes while IFT trains U-turn at the tip.Journal of cell science · 2021Article
- Live-cell fluorescence imaging of ciliary dynamics.Biophysics reports · 2021Article
- Diffusion rather than intraflagellar transport likely provides most of the tubulin required for axonemal assembly inJournal of cell science · 2020Article
- The BBSome restricts entry of tagged carbonic anhydrase 6 into the cis-flagellum of Chlamydomonas reinhardtii.PloS one · 2020Article
- In vivo analysis of outer arm dynein transport reveals cargo-specific intraflagellar transport properties.Molecular biology of the cell · 2018Article
- In vivo analyses of radial spoke transport, assembly, repair and maintenance.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Primary cilia proteins: ciliary and extraciliary sites and functions.Cellular and molecular life sciences : CMLS · 2018Review
- The IDA3 adapter, required for intraflagellar transport of I1 dynein, is regulated by ciliary length.Molecular biology of the cell · 2018Article
- The Bardet-Biedl syndrome protein complex is an adapter expanding the cargo range of intraflagellar transport trains for ciliary export.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
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Authors and funding
1 author.
Funding
Abstract
The assembly of cilia and eukaryotic flagella (interchangeable terms) requires the import of numerous proteins from the cell body into the growing organelle. Proteins move into and inside cilia by diffusion and by motor-based intraflagellar transport (IFT). Many aspects of ciliary protein transport such as the distribution of unloading sites and the frequency of transport can be analyzed using direct in vivo imaging of fluorescently tagged proteins. Here, we will describe how to use total internal reflection fluorescence microcopy (TIRFM) to analyze protein transport in the flagella of the unicellular alga Chlamydomonas reinhardtii, a widely used model for cilia and cilia-related disease.
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