ArticleBMC anesthesiology2016
shRNA mediated knockdown of Nav1.7 in rat dorsal root ganglion attenuates pain following burn injury.
Article in BMC anesthesiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Genetic identification of mouse trigeminal afferents responsible for mechanical allodynia.Cell reports · 2026Article
- Review
- Preemptive analgesia regimen modulates hippocampalJournal of Taibah University Medical Sciences · 2025Article
- Voltage-gated sodium channels in excitable cells as drug targets.Nature reviews. Drug discovery · 2025Review
- Disordered but effective: short linear motifs as gene therapy targets for hyperexcitability disorders.The Journal of clinical investigation · 2024Article
- Fibroblast growth factor homologous factor 2 attenuates excitability of DRG neurons.Journal of neurophysiology · 2022Article
- Chemical and Biological Tools for the Study of Voltage-Gated Sodium Channels in Electrogenesis and Nociception.Chembiochem : a European journal of chemical biology · 2022Review
- Developing nociceptor-selective treatments for acute and chronic pain.Science translational medicine · 2021Article
- Nociceptor Overexpression of NaThe journal of pain · 2021Article
- TNF-α - mediated peripheral and central inflammation are associated with increased incidence of PND in acute postoperative pain.BMC anesthesiology · 2021Article
- Long-lasting analgesia via targeted in situ repression of NaScience translational medicine · 2021Article
- Overexpressed NaFrontiers in cellular neuroscience · 2021Article
- Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro.Bioengineering (Basel, Switzerland) · 2020Article
- Influence of androgenic blockade with flutamide on pain behaviour and expression of the genes that encode the NaV1.7 and NaV1.8 voltage-dependent sodium channels in a rat model of postoperative pain.Journal of translational medicine · 2019Article
- Mining the NaBiochemical pharmacology · 2019Review
- Gene therapy for neurological disorders: progress and prospects.Nature reviews. Drug discovery · 2018Review
- The NAJournal of molecular medicine (Berlin, Germany) · 2018Article
- Melatonin Suppresses Neuropathic Pain via MT2-Dependent and -Independent Pathways in Dorsal Root Ganglia Neurons of Mice.Theranostics · 2017Article
- Targeting dorsal root ganglia and primary sensory neurons for the treatment of chronic pain: an update.Expert opinion on therapeutic targetsReview
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAbnormal acute pain after burn injury still torments patients severely. In this study, we investigated that one voltage gated sodium channel Nav1.7 plays a vital role in lowering heat pain threshold after burn injury, and the hypothesis that knockdown of Nav1.7 attenuates pain following burn injury.
methodsSixty eight adult male Sprague-Dawley rats were divided into 4 treatment groups: (1) sham, which hind paw was put on the room temperature metal plate for 15 s (2) burn model, which hind paw was put on the 85 °C metal plate for 15 s. (3) Burn injury + lentiviral vector -SCN9AsiRNA-GFP (LV- SCN9AsiRNA-GFP group, n = 18), which receive the DRG microinjection of LV- SCN9AsiRNA-GFP on the zero day. (4) Burn injury + lentiviral vector negative control (LV-NC-GFP group, n = 18), which receive the DRG microinjection of empty lentiviral vector on the zero day.
resultsBoth mechanical and heat threshold were measured from day 1 to 21. Meanwhile, expression of sodium channels Nav1.7 in injured dorsal root ganglia were measured on post-operative days 7(POD 7). Rats exhibited decreased thresholds on both mechanical allodynia and thermal withdrawl latency, accompanied by increased Nav1.7 and c-fos expression in dorsal root ganglion (DRG). And knockdown of Nav1.7 in L5DRG led to the attenuation of burn injury-induced mechanical allodynia and thermal hyperalgesia in the rats.
conclusionWe provide evidence that shRNA mediated knockdown of Nav1.7 attenuates burn induced pain in rats as well as decreased the activiation of c-fos protein.
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