ArticleBMC genomics2016
Transcriptional profiling analysis of Penicillium digitatum, the causal agent of citrus green mold, unravels an inhibited ergosterol biosynthesis pathway in response to citral.
Article in BMC genomics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 120 citations in OpenAlex.
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- Inhibitory Properties of Cinnamon Bark Oil against Postharvest PathogenJournal of fungi (Basel, Switzerland) · 2024Article
- Plant secondary metabolite citral interferes with Phytophthora capsici virulence by manipulating the expression of effector genes.Molecular plant pathology · 2023Article
- Transcriptomic Profile of Penicillium digitatum Reveals Novel Aspects of the Mode of Action of the Antifungal Protein AfpB.Microbiology spectrum · 2023Article
- Protection of postharvest grains from fungal spoilage by biogenic volatiles.Applied microbiology and biotechnology · 2023Review
- Iturin A Strongly Inhibits the Growth and T-2 Toxin Synthesis ofFoods (Basel, Switzerland) · 2023Article
- Hinokitiol inhibitsFrontiers in microbiology · 2023Article
- Antifungal Activity of Essential Oil and Plant-Derived Natural Compounds againstAntibiotics (Basel, Switzerland) · 2022Review
- Mechanisms underlying the inhibitory effects of linalool on Aspergillus flavus spore germination.Applied microbiology and biotechnology · 2022Article
- Direct and Indirect Effects of Essential Oils for Sustainable Crop Protection.Plants (Basel, Switzerland) · 2022Review
- Effect of Gaseous Citral on Table Grapes Contaminated byFoods (Basel, Switzerland) · 2022Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundGreen mold caused by Penicillium digitatum is the most damaging postharvest diseases of citrus fruit. Previously, we have observed that citral dose-dependently inhibited the mycelial growth of P. digitatum, with the minimum inhibitory concentration (MIC) of 1.78 mg/mL, but the underlying molecular mechanism is barely understood.
resultsIn this study, the transcriptional profiling of the control and 1/2MIC-citral treated P. digitatum mycelia after 30 min of exposure were analyzed by RNA-Seq. A total of 6355 genes, including 2322 up-regulated and 4033 down-regulated genes, were found to be responsive to citral. These genes were mapped to 155 KEGG pathways, mainly concerning mRNA surveillance, RNA polymerase, RNA transport, aminoacyl-tRNA biosynthesis, ABC transporter, glycolysis/gluconeogenesis, citrate cycle, oxidative phosphorylation, sulfur metabolism, nitrogen metabolism, inositol phosphate metabolism, fatty acid biosynthesis, unsaturated fatty acids biosynthesis, fatty acid metabolism, and steroid biosynthesis. Particularly, citral exposure affected the expression levels of five ergosterol biosynthetic genes (e.g. ERG7, ERG11, ERG6, ERG3 and ERG5), which corresponds well with the GC-MS results, the reduction in ergosterol content, and accumulation of massive lanosterol. In addition, ERG11, the gene responsible for lanosterol 14α-demethylase, was observed to be the key down-regulated gene in response to citral.
conclusionOur present finding suggests that citral could exhibit its antifungal activity against P. digitatum by the down-regulation of ergosterol biosynthesis.
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