ArticlePloS one2016
Selection of Suitable Reference Genes for RT-qPCR Normalization under Abiotic Stresses and Hormone Stimulation in Persimmon (Diospyros kaki Thunb).
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Advancements in Reference Gene Selection for Fruit Trees: A Comprehensive Review.International journal of molecular sciences · 2024Review
- Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions.PloS one · 2024Article
- A qPCR Method to Assay Endonuclease Activity of Cas9-sgRNA Ribonucleoprotein Complexes.Journal of microbiology and biotechnology · 2023Article
- Article
- Physiological Changes and Transcriptomic Analysis throughout On-Tree Fruit Ripening Process in Persimmon (Plants (Basel, Switzerland) · 2023Article
- Evaluating and Validating Sunflower Reference Genes for Q-PCR Studies Under High Temperature Condition.Iranian journal of biotechnology · 2023Article
- Intra and Inter-specific Variability of Salt Tolerance Mechanisms inFrontiers in plant science · 2020Article
- A cross population between D. kaki and D. virginiana shows high variability for saline tolerance and improved salt stress tolerance.PloS one · 2020Article
- Selection of Reference Genes for qPCR Analyses of Gene Expression in Ramie Leaves and Roots across Eleven Abiotic/Biotic Treatments.Scientific reports · 2019Article
- Postmenopausal Osteoporosis reference genes for qPCR expression assays.Scientific reports · 2019Article
- Article
- Selection of reference genes for qPCR normalization in buffalobur (Solanum rostratum Dunal).Scientific reports · 2019Article
- Selection of reliable reference genes for quantitative RT-PCR in garlic under salt stress.PeerJ · 2019Article
- Transcriptomic analysis of interstock-induced dwarfism in Sweet Persimmon (Horticulture research · 2019Article
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8 authors.
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Abstract
The success of quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) to quantify gene expression depends on the stability of the reference genes used for data normalization. To date, systematic screening for reference genes in persimmon (Diospyros kaki Thunb) has never been reported. In this study, 13 candidate reference genes were cloned from 'Nantongxiaofangshi' using information available in the transcriptome database. Their expression stability was assessed by geNorm and NormFinder algorithms under abiotic stress and hormone stimulation. Our results showed that the most suitable reference genes across all samples were UBC and GAPDH, and not the commonly used persimmon reference gene ACT. In addition, UBC combined with RPII or TUA were found to be appropriate for the "abiotic stress" group and α-TUB combined with PP2A were found to be appropriate for the "hormone stimuli" group. For further validation, the transcript level of the DkDREB2C homologue under heat stress was studied with the selected genes (CYP, GAPDH, TUA, UBC, α-TUB, and EF1-α). The results suggested that it is necessary to choose appropriate reference genes according to the test materials or experimental conditions. Our study will be useful for future studies on gene expression in persimmon.
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