ArticleACS nano2016
DNA Translocation through Nanopores at Physiological Ionic Strengths Requires Precise Nanoscale Engineering.
Article in ACS nano, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
23 citing papers in PubMed.
- Bioinformatic and computational biophysics tools for nanopore engineering: a review from standard approaches to machine learning advancements.Journal of nanobiotechnology · 2026Review
- Background-Free Nanopore Decoding of microRNA Expression Patterns Using Circular Diagnostic DNA.Analytical chemistry · 2026Article
- Hetero-Oligomeric Protein Pores for Single-Molecule Sensing.The Journal of membrane biology · 2025Review
- Redesign of Translocon EXP2 Nanopore for Detecting Peptide Fragments.Small methods · 2025Article
- Large and Stable Nanopores Formed by Complement Component 9 for Characterizing Single Folded Proteins.ACS nano · 2025Article
- Structure and Dynamics of dsDNA in Cell-like Environments.Entropy (Basel, Switzerland) · 2022Review
- Unbiased Data Analysis for the Parameterization of Fast Translocation Events through Nanopores.ACS omega · 2022Article
- Control of subunit stoichiometry in single-chain MspA nanopores.Biophysical journal · 2022Article
- Review
- Autonomous and Active Transport Operated by an Entropic DNA Piston.Nano letters · 2021Article
- Biological Nanopores: Engineering on Demand.Life (Basel, Switzerland) · 2021Review
- Influence of Electrolyte Concentration on Single-Molecule Sensing of Perfluorocarboxylic Acids.Frontiers in chemistry · 2021Article
- Electro-Osmotic Vortices Promote the Capture of Folded Proteins by PlyAB Nanopores.Nano letters · 2020Article
- Giant single molecule chemistry events observed from a tetrachloroaurate(III) embedded Mycobacterium smegmatis porin A nanopore.Nature communications · 2019Article
- Engineering and Modeling the Electrophoretic Trapping of a Single Protein Inside a Nanopore.ACS nano · 2019Article
- Electrode-free nanopore sensing by DiffusiOptoPhysiology.Science advances · 2019Article
- Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications.International journal of molecular sciences · 2019Review
- γ-Hemolysin Nanopore Is Sensitive to Guanine-to-Inosine Substitutions in Double-Stranded DNA at the Single-Molecule Level.Journal of the American Chemical Society · 2018Article
- Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores.Nature communications · 2017Article
- Single-molecule nanopore enzymology.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Many important processes in biology involve the translocation of a biopolymer through a nanometer-scale pore. Moreover, the electrophoretic transport of DNA across nanopores is under intense investigation for single-molecule DNA sequencing and analysis. Here, we show that the precise patterning of the ClyA biological nanopore with positive charges is crucial to observe the electrophoretic translocation of DNA at physiological ionic strength. Surprisingly, the strongly electronegative 3.3 nm internal constriction of the nanopore did not require modifications. Further, DNA translocation could only be observed from the wide entry of the nanopore. Our results suggest that the engineered positive charges are important to align the DNA in order to overcome the entropic and electrostatic barriers for DNA translocation through the narrow constriction. Finally, the dependencies of nucleic acid translocations on the Debye length of the solution are consistent with a physical model where the capture of double-stranded DNA is diffusion-limited while the capture of single-stranded DNA is reaction-limited.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.