ArticlePLoS genetics2016
The Tumor-Associated Variant RAD51 G151D Induces a Hyper-Recombination Phenotype.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- The BRCA2 R2645G variant increases DNA binding and induces hyper-recombination.Nucleic acids research · 2024Article
- Sequential requirements for distinct Polθ domains during theta-mediated end joining.Molecular cell · 2024Article
- RAD51 bypasses the CMG helicase to promote replication fork reversal.Science (New York, N.Y.) · 2023Article
- Transgenic mice harboring direct repeat substrates reveal key underlying causes of homologous recombination in vivo.DNA repair · 2022Review
- Article
- RAD51AP1 and RAD54L Can Underpin Two Distinct RAD51-Dependent Routes of DNA Damage RepairFrontiers in cell and developmental biology · 2022Article
- Article
- UGM: a more stable procedure for large-scale multiple testing problems, new solutions to identify oncogene.Theoretical biology & medical modelling · 2019Review
- Development of hRad51-Cas9 nickase fusions that mediate HDR without double-stranded breaks.Nature communications · 2019Article
- Article
- Article
- Human RAD51 rapidly forms intrinsically dynamic nucleoprotein filaments modulated by nucleotide binding state.Nucleic acids research · 2018Article
- Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations.Nature cell biology · 2018Article
- Variation in RAD51 details a hub of functions: opportunities to advance cancer diagnosis and therapy.F1000Research · 2018Review
- Defects in recombination activity caused by somatic and germline mutations in the multimerization/BRCA2 binding region of human RAD51 protein.DNA repair · 2017Article
- A cell-penetrating antibody inhibits human RAD51 via direct binding.Nucleic acids research · 2017Article
- The NEIL1 G83D germline DNA glycosylase variant induces genomic instability and cellular transformation.Oncotarget · 2017Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
The RAD51 protein plays a key role in the homology-directed repair of DNA double-strand breaks and is important for maintaining genome stability. Here we report on a novel human RAD51 variant found in an aggressive and therapy-refractive breast carcinoma. Expression of the RAD51 G151D variant in human breast epithelial cells increases the levels of homology-directed repair. Expression of RAD51 G151D in cells also promotes high levels of chromosomal aberrations and sister chromatid exchanges. In vitro, the purified RAD51 G151D protein directly and significantly enhances DNA strand exchange activity in the presence of RPA. In concordance with this result, co-incubation of G151D with BRCA2 resulted in a much higher level of strand-exchange activity compared to WT RAD51. Strikingly, the RAD51 G151D variant confers resistance to multiple DNA damaging agents, including ionizing radiation, mitomycin C, and doxorubicin. Our findings demonstrate that the RAD51 G151D somatic variant has a novel hyper-recombination phenotype and suggest that this property of the protein is important for the repair of DNA damage, leading to drug resistance.
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