ArticlePlant molecular biology2016
De novo transcriptome analysis reveals insights into dynamic homeostasis regulation of somatic embryogenesis in upland cotton (G. hirsutum L.).
Article in Plant molecular biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 40 citations in OpenAlex.
- Evolution and Expression Analysis of PAO Gene Family in Cotton: Focusing on Fiber Development and Stress Response.Plants (Basel, Switzerland) · 2026Article
- Transcriptome Analysis Revealed the Regulatory Mechanism of DIMBOA Affecting Early Somatic Embryogenesis inPlants (Basel, Switzerland) · 2025Article
- Transcriptome and Gene Expression Analysis RevealedInternational journal of molecular sciences · 2024Article
- Multi-omics analysis reveals the positive impact of differential chloroplast activity during in vitro regeneration of barley.Plant molecular biology · 2024Article
- Proteomic and Phosphoproteomic Analyses during Plant Regeneration Initiation in Cotton (Genes · 2024Article
- Physiological, Metabolic, and Transcriptomic Analyses Reveal Mechanisms of Proliferation and Somatic Embryogenesis of Litchi (International journal of molecular sciences · 2024Article
- Transcriptome Analysis of Embryogenic and Non-Embryogenic Callus ofCurrent issues in molecular biology · 2023Article
- Endogenous auxin maintains embryonic cell identity and promotes somatic embryo development in Arabidopsis.The Plant journal : for cell and molecular biology · 2023Article
- One-Week Scutellar Somatic Embryogenesis in the MonocotPlants (Basel, Switzerland) · 2022Article
- Dynamic Transcriptome Analysis Reveals Complex Regulatory Pathway Underlying Induction and Dose Effect by Different Exogenous Auxin IAA and 2,4-D DuringFrontiers in plant science · 2022Article
- Transcriptional Landscape of Cotton Fiber Development and Its Alliance With Fiber-Associated Traits.Frontiers in plant science · 2022Article
- Embryogenic Calli Induction and Salt Stress Response Revealed by RNA-Seq in Diploid Wild SpeciesFrontiers in plant science · 2021Article
- miRNAs as key regulators via targeting the phytohormone signaling pathways during somatic embryogenesis of plants.3 Biotech · 2020Review
- Transcriptome analyses provide insights into the homeostatic regulation of axillary buds in upland cotton (G. hirsutum L.).BMC plant biology · 2020Article
- Article
- Current Perspectives on the Auxin-Mediated Genetic Network that Controls the Induction of Somatic Embryogenesis in Plants.International journal of molecular sciences · 2020Review
- Dynamic Transcriptome Analysis Reveals Uncharacterized Complex Regulatory Pathway Underlying Dose IBA-Induced Embryogenic Redifferentiation in Cotton.International journal of molecular sciences · 2020Article
- Article
- Metabolome and Transcriptome Association Analysis Reveals Dynamic Regulation of Purine Metabolism and Flavonoid Synthesis in Transdifferentiation during Somatic Embryogenesis in Cotton.International journal of molecular sciences · 2019Article
- Dynamic TMT-Based Quantitative Proteomics Analysis of Critical Initiation Process of Totipotency during Cotton Somatic Embryogenesis Transdifferentiation.International journal of molecular sciences · 2019Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant regeneration via somatic embryogenesis (SE) is the key step for genetic improvement of cotton (Gossypium hirsutum L.) through genetic engineering mediated by Agrobacteria, but the molecular mechanisms underlying SE in cotton is still unclear. Here, RNA-Sequencing was used to analyze the genes expressed during SE and their expression dynamics using RNAs isolated from non-embryogenic callus (NEC), embryogenic callus (EC) and somatic embryos (SEs). A total of 101, 670 unigenes were de novo assembled. The genes differentially expressed (DEGs) amongst NEC, EC and SEs were identified, annotated and classified. More DEGs were found between SEs and EC than between EC and NEC. A significant number of DEGs were related to hormone homeostasis, stress and ROS responses, and metabolism of polyamines. To confirm the expression dynamics of selected DEGs involved in various pathways, experiments were set up to investigate the effects of hormones (Indole-3-butytric acid, IBA; Kinetin, KT), polyamines, H2O2 and stresses on SE. Our results showed that exogenous application of IBA and KT positively regulated the development of EC and SEs, and that polyamines and H2O2 promoted the conversion of EC into SEs. Furthermore, we found that low and moderate stress is beneficial for proliferation of EC and SEs formation. Together, our global analysis of transcriptomic dynamics reveals that hormone homeostasis, polyamines, and stress response synergistically regulating SE in cotton.
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