ArticleCritical care (London, England)2016
Impaired Ca(2+) release contributes to muscle weakness in a rat model of critical illness myopathy.
Article in Critical care (London, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- 'Cells · 2026Article
- Protein nutrition in the ICU: a Delphi exercise to highlight knowledge and opinions of different professional groups involved in patient critical care.BMC nutrition · 2026Article
- Evaluating skeletal muscle wasting and weakness in models of critical illness.Clinical science (London, England : 1979) · 2025Review
- Targeting ryanodine receptors with allopurinol and xanthine derivatives for the treatment of cardiac and musculoskeletal weakness disorders.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- A scoping review of preclinical intensive care unit-acquired weakness models.Frontiers in physiology · 2024Article
- Quantum dot-based thermometry uncovers decreased myosin efficiency in an experimental intensive care unit model.Frontiers in physiology · 2024Article
- Mitochondrial Dysfunction in Intensive Care Unit-Acquired Weakness and Critical Illness Myopathy: A Narrative Review.International journal of molecular sciences · 2023Review
- Sphingomyelinase activity promotes atrophy and attenuates force in human muscle fibres and is elevated in heart failure patients.Journal of cachexia, sarcopenia and muscle · 2022Article
- Electrical stimulated GLUT4 signalling attenuates critical illness-associated muscle wasting.Journal of cachexia, sarcopenia and muscle · 2022Article
- Anshen Buxin Liuwei Pill, a Mongolian Medicinal Formula, Could Protect HEvidence-based complementary and alternative medicine : eCAM · 2022Article
- Impact of prolonged sepsis on neural and muscular components of muscle contractions in a mouse model.Journal of cachexia, sarcopenia and muscle · 2021Article
- Measurement of excitation-contraction coupling time in lower extremities.Physical therapy research · 2021Article
- Chaperone co-inducer BGP-15 mitigates early contractile dysfunction of the soleus muscle in a rat ICU model.Acta physiologica (Oxford, England) · 2020Article
- Calcium Fluxes in Work-Related Muscle Disorder: Implications from a Rat Model.BioMed research international · 2019Article
- ICU-acquired weakness: should medical sovereignty belong to any specialist?Critical care (London, England) · 2018Article
- Sweroside Alleviated Aconitine-Induced Cardiac Toxicity in H9c2 Cardiomyoblast Cell Line.Frontiers in pharmacology · 2018Article
- Electrical Stimulation Prevents Preferential Skeletal Muscle Myosin Loss in Steroid-Denervation Rats.Frontiers in physiology · 2018Article
- Mechanical isolation, and measurement of force and myoplasmic free [CaNature protocols · 2017Article
- Mechanism of ICU-acquired weakness: muscle contractility in critical illness.Intensive care medicine · 2017Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCritical illness myopathy is an acquired skeletal muscle disorder with severe myosin loss and muscle weakness frequently seen in intensive care unit (ICU) patients. It is unknown if impaired excitation-contraction coupling contributes to the muscle weakness.
methodsWe used a unique ICU model where rats were deeply sedated, post-synaptically pharmacologically paralyzed, mechanically ventilated and closely monitored for up to ten days. Single intact fibers from the flexor digitorum brevis muscle were isolated and used to measure force and free myoplasmic [Ca(2+)] ([Ca(2+)]i) during tetanic contractions.
resultsFibers from ICU rats had 80 % lower tetanic [Ca(2+)]i and produced only 15 % of the force seen in fibers from sham-operated (SHAM) rats. In the presence of 5 mM caffeine, tetanic [Ca(2+)]i was similar in fibers from ICU and SHAM rats but force was 50 % lower in fibers from ICU rats than SHAM rats. Confocal imaging showed disrupted tetanic [Ca(2+)]i transients in fibers from ICU rats compared to SHAM rats. Western blots showed similar levels of Na(+) channel and dihydropyridine receptor (DHPR) protein expression, whereas ryanodine receptor (RyR) and sarco-endoplasmic reticulum Ca(2+) ATPase 1 (SERCA1) expression was markedly lower in muscle of ICU rats than in SHAM rats. Immunohistochemical analysis showed that distribution of Na(+) channel and DHPR protein on the sarcolemma was disrupted in fibers from ICU rats compared with SHAM rats.
conclusionsThese results suggest that impaired SR Ca(2+) release contributes to the muscle weakness seen in patients in ICU.
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