ArticleSoft matter2016
cDICE method produces giant lipid vesicles under physiological conditions of charged lipids and ionic solutions.
Article in Soft matter, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 70 citations in OpenAlex.
- Quantification of cholesterol incorporation in giant unilamellar vesicles produced by a modified cDICE method.Soft matter · 2026Article
- Engineering synthetic cells with intramembrane domains possessing distinct bilayer asymmetries.Nature communications · 2026Article
- Light-induced assembly and repeatable actuation in CaNature communications · 2026Article
- Strategy for Generating Giant Unilamellar Vesicles with Tunable Size Using the Modified cDICE Method.ACS synthetic biology · 2025Article
- Challenges in observing transcription-translation for bottom-up synthetic biology.QRB discovery · 2025Review
- Article
- Several common methods of making vesicles (except an emulsion method) capture intended lipid ratios.Biophysical journal · 2024Article
- Effect of leaflet asymmetry on the stretching elasticity of lipid bilayers with phosphatidic acid.Biophysical journal · 2024Article
- Artificial cells for in vivo biomedical applications through red blood cell biomimicry.Nature communications · 2024Review
- An Efficient Method for the Production of High-Purity Bioinspired Large Unilamellar Vesicles.ACS synthetic biology · 2024Article
- Lipid vesicle-based molecular robots.Lab on a chip · 2024Review
- Minimal Out-of-Equilibrium Metabolism for Synthetic Cells: A Membrane Perspective.ACS synthetic biology · 2023Review
- Function Investigations and Applications of Membrane Proteins on Artificial Lipid Membranes.International journal of molecular sciences · 2023Review
- DisGUVery: A Versatile Open-Source Software for High-Throughput Image Analysis of Giant Unilamellar Vesicles.ACS synthetic biology · 2023Article
- Effects of cholesterol on the size distribution and bending modulus of lipid vesicles.PloS one · 2022Article
- Light-Triggered Cargo Loading and Division of DNA-Containing Giant Unilamellar Lipid Vesicles.Nano letters · 2021Article
- Optimized cDICE for Efficient Reconstitution of Biological Systems in Giant Unilamellar Vesicles.ACS synthetic biology · 2021Article
- Direct imaging of liquid domains in membranes by cryo-electron tomography.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Influence of cholesterol on electroporation in lipid membranes of giant vesicles.European biophysics journal : EBJ · 2020Article
- Development of Artificial Cell Models Using Microfluidic Technology and Synthetic Biology.Micromachines · 2020Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Giant unilamellar vesicles are a powerful and common tool employed in biophysical studies of lipid membranes. Here we evaluate a recently introduced method of vesicle formation, "continuous droplet interface crossing encapsulation" (cDICE). This method produces monodisperse giant unilamellar vesicles of controlled sizes and high encapsulation efficiencies, using readily available instrumentation. We find that mixtures of phospholipids within vesicle membranes produced by cDICE undergo phase separation at the same characteristic temperatures as lipids in vesicles formed by a complementary technique. We find that the cDICE method is effective both when vesicles are produced from charged lipids and when the surrounding buffer contains a high concentration of salt. A shortcoming of the technique is that cholesterol is not substantially incorporated into vesicle membranes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.