Effects of Surface Epitope Coverage on the Sensitivity of Displacement Assays that Employ Modified Nanoparticles: Using Bisphenol A as a Model Analyte.

Yang Lu, Joshua Richard Peterson, Erwann Luais, John Justin Gooding, Nanju Alice Lee

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Article in Biosensors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed

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1 citing paper in PubMed.

Measurement of Low Concentration of Micro-Plastics by Detection of Bioaffinity-Induced Particle Retention Using Surface Plasmon Resonance Biosensors.Biosensors · 2021
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5 · Who and what money

Authors and funding

5 authors.

Yang LuSchool of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia. luyang@tust.edu.cn.

Joshua Richard PetersonSchool of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia. peterson.j@unsw.edu.au.

Erwann LuaisSchool of Chemistry, University of New South Wales, Sydney, NSW 2052, Australia. erwann.luais@univ-tours.fr.

John Justin GoodingSchool of Chemistry and Australian Centre for NanoMedicine, University of New South Wales, Sydney, NSW 2052, Australia. justin.gooding@unsw.edu.au.

Nanju Alice LeeSchool of Chemical Engineering and ARC Training Centre for Advanced Technologies in Food Manufacture, University of New South Wales, Sydney, NSW 2052, Australia. alice.lee@unsw.edu.au.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

With the ever-increasing use of nanoparticles in immunosensors, a fundamental study on the effect of epitope density is presented herein, with a small molecule epitope, on the performance of the displacement assay format in an enzyme-linked immunosorbent assay (ELISA). Thiolated bisphenol A (BPA) functionalized gold nanoparticles (cysBPAv-AuNPs) and specific anti-BPA antibodies are employed for this purpose. It is shown that the displacement of cysBPAv-AuNPs bound to the immobilized antibodies was influenced by both the avidity of bound cysBPAv-AuNPs and the concentration of free BPA to displace it. The importance of surface epitope density was that it changed the number of epitopes in close proximity to the antibody-binding site. This then influenced the avidity of cysBPAv-AuNPs bound to the immobilized antibody. Furthermore, the molar epitope concentration in an assay appears to affect the degree of antibody binding site saturation. Controlling surface epitope density of the functionalized nanoparticles and molar epitope concentration in an assay leads to a decrease of the concentration of free BPA required to displace the bound cysBPAv-AuNP, and hence better assay performance with regards to the D50 value and dynamic range in the displacement assay.

Indexed as

Benzhydryl CompoundsBiosensing TechniquesEpitopesNanoparticlesPhenolsAntibodies, ImmobilizedBisphenol A CompoundsEnzyme-Linked Immunosorbent AssayGoldMetal NanoparticlesSensitivity and SpecificityAntibodies, ImmobilizedBenzhydryl Compoundsbisphenol ABisphenol A CompoundsEpitopesGoldPhenolsbisphenol Adisplacement ELISAgold nanoparticlessurface epitope density

Identifiers

PMID27509530

DOI10.3390/bios6030043

PMCPMC5039662

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LicenceCC BY

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.