ArticleStem cell reports2016
Embryonic Stem Cell Culture Conditions Support Distinct States Associated with Different Developmental Stages and Potency.
Article in Stem cell reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 64 citations in OpenAlex.
- Naïve pluripotency and genomic stability are coordinated in embryonic stem cells by a novel pluripotency regulator ZFP998.Nucleic acids research · 2026Article
- African pygmy mouse iPSCs as a model for in vitro embryogenesis, interspecies chimerism, and blastocyst complementation.Cell reports methods · 2026Article
- Addressing the specific roles of histone modifications in transcriptional repression.Nature communications · 2025Article
- Altering metabolism programs cell identity via NADThe EMBO journal · 2025Article
- Deep learning-based models for preimplantation mouse and human embryos based on single-cell RNA sequencing.Nature methods · 2025Article
- Transcription factor co-expression mediates lineage priming for embryonic and extra-embryonic differentiation.Stem cell reports · 2024Article
- Impact of eIF2α phosphorylation on the translational landscape of mouse embryonic stem cells.Cell reports · 2024Article
- Lethal Phenotype-Based Database Screening Identifies Ceramide as a Negative Regulator of Primitive Streak Formation.Stem cells (Dayton, Ohio) · 2023Article
- Grainyhead-like 2 is required for morphological integrity of mouse embryonic stem cells and orderly formation of inner ear-like organoids.Frontiers in cell and developmental biology · 2023Article
- Comparison of lncRNA Expression in the Uterus between Periods of Embryo Implantation and Labor in Mice.Animals : an open access journal from MDPI · 2022Article
- Chromatin as a sensor of metabolic changes during early development.Frontiers in cell and developmental biology · 2022Review
- Transcriptome analysis reveals high tumor heterogeneity with respect to re-activation of stemness and proliferation programs.PloS one · 2022Article
- Article
- Distinct roles of haspin in stem cell division and male gametogenesis.Scientific reports · 2021Article
- Cooperation between HDAC3 and DAX1 mediates lineage restriction of embryonic stem cells.The EMBO journal · 2021Article
- ACK1 is dispensable for development, skin tumor formation, and breast cancer cell proliferation.FEBS open bio · 2021Article
- All models are wrong, but some are useful: Establishing standards for stem cell-based embryo models.Stem cell reports · 2021Review
- CRISPR/Cas9: targeted genome editing for the treatment of hereditary hearing loss.Journal of applied genetics · 2020Review
- Current status of cardiac regenerative medicine; An update on point of view to cell therapy application.Journal of cardiovascular and thoracic research · 2020Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Embryonic stem cells (ESCs) are cell lines derived from the mammalian pre-implantation embryo. Here we assess the impact of derivation and culture conditions on both functional potency and ESC transcriptional identity. Individual ESCs cultured in either two small-molecule inhibitors (2i) or with knockout serum replacement (KOSR), but not serum, can generate high-level chimeras regardless of how these cells were derived. ESCs cultured in these conditions showed a transcriptional correlation with early pre-implantation embryos (E1.5-E3.5) and contributed to development from the 2-cell stage. Conversely, the transcriptome of serum-cultured ESCs correlated with later stages of development (E4.5), at which point embryonic cells are more restricted in their developmental potential. Thus, ESC culture systems are not equivalent, but support cell types that resemble distinct developmental stages. Cells derived in one condition can be reprogrammed to another developmental state merely by adaptation to another culture condition.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.