ArticleBiophysical journal2016
Phasor Analysis of Local ICS Detects Heterogeneity in Size and Number of Intracellular Vesicles.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Multimodal Phasor Analysis for Digital Pathology: Quantitative Characterization of Liver Iron Overload.Chemical & biomedical imaging · 2026Article
- ESPRESSO: spatiotemporal omics based on organelle phenotyping.Nature methods · 2025Article
- Multimodal Phasor Approach to Study Breast Cancer Cell Invasion in a 3D Spheroid Model.Chemical & biomedical imaging · 2025Article
- Quantitative analysis of lipofuscin in neurodegenerative diseases using serial sectioning two-photon microscopy and fluorescence lifetime imaging microscopy.Neurophotonics · 2025Article
- Particle-based phasor-FLIM-FRET resolves protein-protein interactions inside single viral particles.Biophysical reports · 2023Article
- Imaging-based study demonstrates how the DEK nanoscale distribution differentially correlates with epigenetic marks in a breast cancer model.Scientific reports · 2023Article
- SPLIT-PIN software enabling confocal and super-resolution imaging with a virtually closed pinhole.Scientific reports · 2023Article
- Alterations induced by the PML-RARα oncogene revealed by image cross correlation spectroscopy.Biophysical journal · 2022Article
- A phasor-based approach to improve optical sectioning in any confocal microscope with a tunable pinhole.Microscopy research and technique · 2022Article
- Measuring Molecular Diffusion in Dynamic Subcellular Nanostructures by Fast Raster Image Correlation Spectroscopy and 3D Orbital Tracking.International journal of molecular sciences · 2022Article
- Novel Tools to Measure Single Molecules Colocalization in Fluorescence Nanoscopy by Image Cross Correlation Spectroscopy.Nanomaterials (Basel, Switzerland) · 2022Article
- Evaluation of sted super-resolution image quality by image correlation spectroscopy (QuICS).Scientific reports · 2021Article
- Phasor approach of Mueller matrix optical scanning microscopy for biological tissue imaging.Biophysical journal · 2021Article
- Chromatin investigation in the nucleus using a phasor approach to structured illumination microscopy.Biophysical journal · 2021Article
- Fluorescence Fluctuation Spectroscopy enables quantification of potassium channel subunit dynamics and stoichiometry.Scientific reports · 2021Article
- Phasor S-FLIM: a new paradigm for fast and robust spectral fluorescence lifetime imaging.Nature methods · 2021Article
- Measuring Nanoscale Distances by Structured Illumination Microscopy and Image Cross-Correlation Spectroscopy (SIM-ICCS).Sensors (Basel, Switzerland) · 2021Article
- Direct Photon-by-Photon Analysis of Time-Resolved Pulsed Excitation Data using Bayesian Nonparametrics.Cell reports. Physical science · 2020Article
- Pre-processing visualization of hyperspectral fluorescent data with Spectrally Encoded Enhanced Representations.Nature communications · 2020Article
- Nanoscale Distribution of Nuclear Sites by Super-Resolved Image Cross-Correlation Spectroscopy.Biophysical journal · 2019Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Organelles represent the scale of organization immediately below that of the cell itself, and their composition, size, and number are tailored to their function. Monitoring the size and number of organelles in live cells is relevant for many applications but can be challenging due to their highly heterogeneous properties. Image correlation spectroscopy is a well-established analysis method capable of extracting the average size and number of particles in images. However, when image correlation spectroscopy is applied to a highly heterogeneous system, it can fail to retrieve, from a single correlation function, the characteristic size and the relative amount associated to each subspecies. Here, we describe a fast, unbiased, and fit-free algorithm based on the phasor analysis of multiple local image correlation functions, capable of mapping the sizes of elements contained in a heterogeneous system. The method correctly provides the size and number of separate subspecies, which otherwise would be hidden in the average properties of a single correlation function. We apply the method to quantify the spatial and temporal heterogeneity in the size and number of intracellular vesicles formed after endocytosis in live cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.